Detailed information of TA system TA194213

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 4705202..4706797
• Replicon: chromosome
• Accession: NZ_CP070505
• Organism: Pseudomonas toyotomiensis strain SM2

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: JWV26_RS21805
• Protein ID: WP_206417806.1
• Coordinates: 4705472..4706797 (-)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A1G5YAZ4
• Locus tag: JWV26_RS21800
• Protein ID: WP_017676963.1
• Coordinates: 4705202..4705471 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JWV26_RS21780	4701126..4701596	+	471	WP_206417803.1	phosphate-starvation-inducible PsiE family protein	-
JWV26_RS21785	4701907..4703688	+	1782	WP_206417804.1	monovalent cation:proton antiporter-2 (CPA2) family protein	-
JWV26_RS21790	4703909..4704877	+	969	WP_206417805.1	TIGR03571 family LLM class oxidoreductase	-
JWV26_RS21795	4704999..4705208	+	210	WP_079783796.1	hypothetical protein	-
JWV26_RS21800	4705202..4705471	-	270	WP_017676963.1	helix-turn-helix domain-containing protein	Antitoxin
JWV26_RS21805	4705472..4706797	-	1326	WP_206417806.1	HipA domain-containing protein	Toxin
JWV26_RS21810	4707050..4707256	+	207	WP_017676961.1	hypothetical protein	-
JWV26_RS21815	4707364..4708506	+	1143	WP_206417807.1	elongation factor P maturation arginine rhamnosyltransferase EarP	-
JWV26_RS21820	4708541..4709113	+	573	WP_003460252.1	elongation factor P	-
JWV26_RS21825	4709213..4710175	-	963	WP_206417808.1	LysR family transcriptional regulator	-
JWV26_RS21830	4710275..4711042	+	768	WP_206417809.1	TSUP family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-380)

Antitoxin

• Cro/C1-type helix-turn-helix domain (25-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 48983.34 Da
• Isoelectric Point: 5.7864

>T194213 WP_206417806.1 NZ_CP070505:c4706797-4705472 [Pseudomonas toyotomiensis]
MHSLTLQLYSGHAWHDAMHLNFEQPEAGLAGACSFGYEQAYLLETLEHIGSRLEHAVSVTVPLDWESHRASEAPAFLHDI
LPAGAARRFLLQRLGDQKPENLGLDLFLLSRCAPAPVGNLRIKEAAAALQESPPLGFSREDVINRDARFLEYAYEQGAAI
GGATGAGGEAPKLLLTEDREGALHPDAALPDESATRHWFVKFARNQARQTDQDILRSEFCYYRAIQRLGLDTVPAEGLAL
EEGHKPSLWMHRFDRHLSAAGVERSAMESLYSLAGVTVPGSYLLHTDALNHLVRLWQKTGQGAQIGDMIFEYLQRDLLNQ
ILGNSDNHGRNTSILRNGTGLKLAPIYDLAPMVMDDEGVTRTTKWPQSIEIAGEVNWRQACAALAQWTDADVLYERLRGA
ALDFLALPDLLSDQGLPNSTMNHPRIALRTLHQRLNRWELV

Nucleotide

• Length: 1326 bp

>T194213 NZ_CP093390:c4097086-4096802 [Salmonella enterica]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGGACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10104.75 Da
• Isoelectric Point: 10.7429

>AT194213 WP_017676963.1 NZ_CP070505:c4705471-4705202 [Pseudomonas toyotomiensis]
MDIIERNRILTEIQTQLASGELTIGQAVRKLRKEITGLQQARFAQMCKLSLRALRQLEHDESNPTVQTLNSVFNPFGMQV
GIVPKSRIQ

Nucleotide

• Length: 270 bp

>AT194213 NZ_CP093390:c4097318-4097076 [Salmonella enterica]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGATA
GTGAAGATGCACTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A1G5YAZ4