Detailed information of TA system TA194184

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 4105612..4106144
• Replicon: chromosome
• Accession: NZ_CP070494
• Organism: Methylomonas sp. EFPC1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: JWZ98_RS18790
• Protein ID: WP_205451339.1
• Coordinates: 4105612..4105935 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: JWZ98_RS18795
• Protein ID: WP_205451340.1
• Coordinates: 4105932..4106144 (-)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JWZ98_RS18775	4100738..4102345	+	1608	WP_205451338.1	nitroreductase family protein	-
JWZ98_RS18780	4102456..4102851	+	396	WP_033159164.1	hypothetical protein	-
JWZ98_RS18785	4102960..4105442	-	2483	Protein_3704	DEAD/DEAH box helicase family protein	-
JWZ98_RS18790	4105612..4105935	-	324	WP_205451339.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JWZ98_RS18795	4105932..4106144	-	213	WP_205451340.1	DUF3018 family protein	Antitoxin
JWZ98_RS18800	4106219..4106782	-	564	WP_205454495.1	hypothetical protein	-
JWZ98_RS18805	4106680..4107417	-	738	WP_205451341.1	restriction endonuclease subunit S	-
JWZ98_RS18810	4107589..4107987	-	399	WP_205451342.1	GIY-YIG nuclease family protein	-
JWZ98_RS18815	4107984..4109438	-	1455	Protein_3710	N-6 DNA methylase	-
JWZ98_RS18820	4109494..4110834	-	1341	WP_205451343.1	tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-105)

Antitoxin

• Antitoxin MazE-like (7-69)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11429.28 Da
• Isoelectric Point: 8.9153

>T194184 WP_205451339.1 NZ_CP070494:c4105935-4105612 [Methylomonas sp. EFPC1]
VRRGDLVTVALQGDLGKPRPALLIQSDLFDAHPSVTILPITSELRDAPLFRIAVAANERTGLSLASQIMVDKPQSVARNK
IGGVIGRIDDETLLSVNRALAVFLGFA

Nucleotide

• Length: 324 bp

>T194184 NZ_CP093379:c442964-442785 [Ignatzschineria rhizosphaerae]
ATGAATAGCAAAGATTTAATAAAGATGATTGAAAAAGATGGATGGTATCTAGTAGCAACAAGAGGTTCACATCATCAATT
CAAACATCCGACCAAATCGGGAAGAGTCACAATCCCTCACCCCAAGAAAGACCTACCGAAAGGAACAGTCAAAAACATAA
TGAAGCAAGCGGGCATCTAG

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 8128.07 Da
• Isoelectric Point: 6.4561

>AT194184 WP_205451340.1 NZ_CP070494:c4106144-4105932 [Methylomonas sp. EFPC1]
MRTVTSARVQKHRIALREAGLRPLQIWVPDTRREGFAEECRRQSLSIQNDAAEQDALNWLETVADHDGWQ

Nucleotide

• Length: 213 bp

>AT194184 NZ_CP093379:c442740-442336 [Ignatzschineria rhizosphaerae]
ATGTTATTTCACTTAGTGATTCATAAAGATGAAGATAGTACCTATGGAGTAACTATCCCATCCTTAGAGGGGGCTTTTTC
TCATGGTGACACATTAGAAGAAGCTGTATTAAACTCAAAAGAAGCAATCCAATTCCATCTTGAAGGAATTCTTGAAGATG
GGATCGAGCCTGATTTTAAACATCTCACATTAGATGAGATCAAGACTAATCCTGACTATCAAGGAGCTGATCTGATCTCC
GTTGATATTGATACATCTGCTTATACACTTAAACCTGAACGATTTAATGTTAGCTGGCCAAGGTATCTCATCAAAGAAGT
AGACCAATATACAACTCAGCATCCTGATACTCGCTCAAGCTTTCTTGCTAAAGCAGCTAAAAAATATATTGAGCAGAATC
CTTAA