Detailed information of TA system TA194172

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/PRK10514-DUF1778
• Location: 1791..2586
• Replicon: plasmid unnamed2
• Accession: NZ_CP070493
• Organism: Methylococcus sp. EFPC2

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: JWZ97_RS19765
• Protein ID: WP_205434861.1
• Coordinates: 2077..2586 (+)
• Length: 170 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: JWZ97_RS19760
• Protein ID: WP_205434860.1
• Coordinates: 1791..2066 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JWZ97_RS19750	6..752	+	747	WP_205434859.1	TIGR02391 family protein	-
JWZ97_RS19755	791..1705	+	915	WP_205434874.1	tyrosine-type recombinase/integrase	-
JWZ97_RS19760	1791..2066	+	276	WP_205434860.1	DUF1778 domain-containing protein	Antitoxin
JWZ97_RS19765	2077..2586	+	510	WP_205434861.1	GNAT family N-acetyltransferase	Toxin
JWZ97_RS19770	2674..2973	+	300	WP_205434862.1	DUF1778 domain-containing protein	-
JWZ97_RS19775	2974..3462	+	489	WP_205434863.1	GNAT family N-acetyltransferase	-
JWZ97_RS19780	3466..3861	+	396	WP_205434864.1	hypothetical protein	-
JWZ97_RS19785	3957..4115	+	159	WP_205434865.1	hypothetical protein	-
JWZ97_RS19790	4251..5585	-	1335	WP_205434866.1	hypothetical protein	-
JWZ97_RS19795	5707..6882	-	1176	WP_205434867.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..25893	25893

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-85)

Sequences

Toxin

Protein

• Length: 170 a.a.
• Molecular weight: 18441.43 Da
• Isoelectric Point: 8.9085

>T194172 WP_205434861.1 NZ_CP070493:2077-2586 [Methylococcus sp. EFPC2]
MEETPKPSIAKLSIEHDPSTFDCGHKALNTFIRLHALPGQQANISQTYVAVVGQTIVGFHTLVVGDVAYDDAPERLAKGL
PRHPVPILLLARLAVDRTWQGKGLGAALVTDAMRRTLQVADIAGVRALLVHAKNEAAKSFYTHLGFEPFLGEPLILYRLL
KDLRVMSNG

Nucleotide

• Length: 510 bp

>T194172 NZ_CP093374:263617-264033 [Salmonella enterica subsp. enterica serovar Infantis]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10411.86 Da
• Isoelectric Point: 8.9520

>AT194172 WP_205434860.1 NZ_CP070493:1791-2066 [Methylococcus sp. EFPC2]
MPTPRHRSEKLDIRITPEAKQILQQAAKQRHTTISQFVLESALDSASAVLAERSRFALNAEQWEAFQAALDAPPRRHPRM
ERLLNEPTILD

Nucleotide

• Length: 276 bp

>AT194172 NZ_CP093374:263390-263620 [Salmonella enterica subsp. enterica serovar Infantis]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA