Detailed information of TA system TA194163

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 518523..519159
• Replicon: chromosome
• Accession: NZ_CP070483
• Organism: Bacillus sp. LJBS06

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: JQX68_RS02610
• Protein ID: WP_003156187.1
• Coordinates: 518809..519159 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: JQX68_RS02605
• Protein ID: WP_003225183.1
• Coordinates: 518523..518804 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQX68_RS02585	514882..515481	-	600	WP_040084115.1	rhomboid family intramembrane serine protease	-
JQX68_RS02590	515576..515941	+	366	WP_014475874.1	holo-ACP synthase	-
JQX68_RS02595	516107..517123	+	1017	WP_080478309.1	outer membrane lipoprotein carrier protein LolA	-
JQX68_RS02600	517238..518407	+	1170	WP_147772929.1	alanine racemase	-
JQX68_RS02605	518523..518804	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
JQX68_RS02610	518809..519159	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
JQX68_RS02615	519274..520098	+	825	WP_014662853.1	RsbT co-antagonist protein RsbRA	-
JQX68_RS02620	520103..520468	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
JQX68_RS02625	520472..520873	+	402	WP_003240352.1	serine/threonine-protein kinase RsbT	-
JQX68_RS02630	520885..521892	+	1008	WP_014662854.1	phosphoserine phosphatase RsbU	-
JQX68_RS02635	521954..522283	+	330	WP_014662855.1	anti-sigma factor antagonist RsbV	-
JQX68_RS02640	522280..522762	+	483	WP_014662856.1	anti-sigma B factor RsbW	-
JQX68_RS02645	522728..523516	+	789	WP_014662857.1	RNA polymerase sigma factor SigB	-
JQX68_RS02650	523516..524115	+	600	WP_014662858.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T194163 WP_003156187.1 NZ_CP070483:518809-519159 [Bacillus sp. LJBS06]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T194163 NZ_CP093373:c4182862-4182494 [Salmonella enterica subsp. enterica serovar Infantis]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAATCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTTGGCATATCTTCAATGATGGTAACAAACGCACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGCCGTTCGCTTACGCGCCTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT194163 WP_003225183.1 NZ_CP070483:518523-518804 [Bacillus sp. LJBS06]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT194163 NZ_CP093373:c4183080-4182859 [Salmonella enterica subsp. enterica serovar Infantis]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGCGCGGTGACGGCGGGCCCTGTCAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGATCTGGCGGATAAATGA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• PDB: 4ME7
• AlphaFold DB: G4NU32