Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4320506..4321160 Replicon chromosome
Accession NZ_CP070482
Organism Escherichia coli strain BTH101

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag JWV29_RS20950 Protein ID WP_000244777.1
Coordinates 4320753..4321160 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JWV29_RS20945 Protein ID WP_000354046.1
Coordinates 4320506..4320772 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JWV29_RS20920 (4315675) 4315675..4316418 + 744 WP_000951964.1 SDR family oxidoreductase -
JWV29_RS20925 (4316475) 4316475..4317908 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
JWV29_RS20930 (4317953) 4317953..4318264 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
JWV29_RS20935 (4318428) 4318428..4319087 + 660 WP_000250274.1 hemolysin III family protein -
JWV29_RS20940 (4319283) 4319283..4320263 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
JWV29_RS20945 (4320506) 4320506..4320772 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JWV29_RS20950 (4320753) 4320753..4321160 + 408 WP_000244777.1 protein YgfX Toxin
JWV29_RS20955 (4321200) 4321200..4321721 - 522 WP_001055874.1 flavodoxin FldB -
JWV29_RS20960 (4321833) 4321833..4322729 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JWV29_RS20965 (4322754) 4322754..4323464 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JWV29_RS20970 (4323470) 4323470..4325203 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T194159 WP_000244777.1 NZ_CP070482:4320753-4321160 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T194159 NZ_CP093373:1983304-1983407 [Salmonella enterica subsp. enterica serovar Infantis]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT194159 WP_000354046.1 NZ_CP070482:4320506-4320772 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT194159 NZ_CP093373:c1983409-1983266 [Salmonella enterica subsp. enterica serovar Infantis]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References