Detailed information of TA system TA194146

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 2455035..2455714
• Replicon: chromosome
• Accession: NZ_CP070482
• Organism: Escherichia coli strain BTH101

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: JWV29_RS12015
• Protein ID: WP_000854672.1
• Coordinates: 2455373..2455714 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: JWV29_RS12010
• Protein ID: WP_000070395.1
• Coordinates: 2455035..2455352 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JWV29_RS11960 (2450082)	2450082..2450945	+	864	WP_001065553.1	GTPase family protein	-
JWV29_RS11965 (2451037)	2451037..2451858	+	822	WP_000197389.1	DUF932 domain-containing protein	-
JWV29_RS11970 (2452075)	2452075..2452266	+	192	Protein_2352	DeoR family transcriptional regulator	-
JWV29_RS11975 (2452262)	2452262..2452489	+	228	WP_001548158.1	protein YpjK	-
JWV29_RS11980 (2452489)	2452489..2452932	+	444	WP_000824223.1	lipoprotein YafY	-
JWV29_RS11985 (2452955)	2452955..2453422	+	468	WP_001547765.1	protein YkfB	-
JWV29_RS11990 (2453499)	2453499..2453738	+	240	WP_000194654.1	DUF905 family protein	-
JWV29_RS11995 (2453836)	2453836..2454294	+	459	WP_000211838.1	antirestriction protein	-
JWV29_RS12000 (2454310)	2454310..2454786	+	477	WP_000811693.1	RadC family protein	-
JWV29_RS12005 (2454795)	2454795..2455016	+	222	WP_000691994.1	DUF987 domain-containing protein	-
JWV29_RS12010 (2455035)	2455035..2455352	+	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
JWV29_RS12015 (2455373)	2455373..2455714	+	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
JWV29_RS12025 (2456286)	2456286..2457539	-	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
JWV29_RS12030 (2457551)	2457551..2458654	-	1104	WP_001285288.1	glutamate 5-kinase	-
JWV29_RS12035 (2458942)	2458942..2459997	+	1056	WP_000749863.1	phosphoporin PhoE	-
JWV29_RS12040 (2460036)	2460036..2460437	-	402	WP_000174689.1	sigma factor-binding protein Crl	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T194146 WP_000854672.1 NZ_CP070482:2455373-2455714 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T194146 NZ_CP093369:c2911406-2911152 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT194146 WP_000070395.1 NZ_CP070482:2455035-2455352 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT194146 NZ_CP093369:c2911654-2911403 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684