Detailed information of TA system TA194145

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 2238334..2238952
• Replicon: chromosome
• Accession: NZ_CP070482
• Organism: Escherichia coli strain BTH101

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: JWV29_RS10905
• Protein ID: WP_001290581.1
• Coordinates: 2238734..2238952 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: S1PTH5
• Locus tag: JWV29_RS10900
• Protein ID: WP_000344800.1
• Coordinates: 2238334..2238708 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JWV29_RS10890 (2233423)	2233423..2234616	+	1194	WP_001295324.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
JWV29_RS10895 (2234639)	2234639..2237788	+	3150	WP_001132469.1	efflux RND transporter permease AcrB	-
JWV29_RS10900 (2238334)	2238334..2238708	+	375	WP_000344800.1	Hha toxicity modulator TomB	Antitoxin
JWV29_RS10905 (2238734)	2238734..2238952	+	219	WP_001290581.1	HHA domain-containing protein	Toxin
JWV29_RS10910 (2239124)	2239124..2239675	+	552	WP_000102564.1	maltose O-acetyltransferase	-
JWV29_RS10915 (2239791)	2239791..2240261	+	471	WP_000136192.1	YlaC family protein	-
JWV29_RS10920 (2240425)	2240425..2241975	+	1551	WP_001310610.1	cyclic-guanylate-specific phosphodiesterase PdeB	-
JWV29_RS10925 (2242017)	2242017..2242370	-	354	WP_000878140.1	DUF1428 domain-containing protein	-
JWV29_RS10935 (2242749)	2242749..2243060	+	312	WP_000409911.1	MGMT family protein	-
JWV29_RS10940 (2243091)	2243091..2243663	-	573	WP_000779842.1	YbaY family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8678.06 Da
• Isoelectric Point: 8.9008

>T194145 WP_001290581.1 NZ_CP070482:2238734-2238952 [Escherichia coli]
MSEKFLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T194145 NZ_CP093369:2899510-2899884 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14557.39 Da
• Isoelectric Point: 4.7395

>AT194145 WP_000344800.1 NZ_CP070482:2238334-2238708 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Nucleotide

• Length: 375 bp

>AT194145 NZ_CP093369:2899053-2899421 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9QBQ5