Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 651154..651985 Replicon chromosome
Accession NZ_CP070482
Organism Escherichia coli strain BTH101

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag JWV29_RS03035 Protein ID WP_000854814.1
Coordinates 651154..651528 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag JWV29_RS03040 Protein ID WP_001285584.1
Coordinates 651617..651985 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JWV29_RS02995 (646550) 646550..647716 + 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
JWV29_RS03000 (647835) 647835..648308 + 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
JWV29_RS03005 (648506) 648506..649564 + 1059 WP_001200891.1 FUSC family protein -
JWV29_RS03010 (649736) 649736..650065 + 330 WP_000450409.1 DUF496 family protein -
JWV29_RS03015 (650166) 650166..650300 - 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
JWV29_RS03020 (650420) 650420..650548 + 129 Protein_593 transposase domain-containing protein -
JWV29_RS03025 (650837) 650837..650917 - 81 Protein_594 hypothetical protein -
JWV29_RS03030 (650963) 650963..651157 - 195 WP_000988600.1 DUF5983 family protein -
JWV29_RS03035 (651154) 651154..651528 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
JWV29_RS03040 (651617) 651617..651985 - 369 WP_001285584.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
JWV29_RS03045 (652059) 652059..652280 - 222 WP_000692323.1 DUF987 domain-containing protein -
JWV29_RS03050 (652343) 652343..652789 - 447 WP_000187523.1 RadC family protein -
JWV29_RS03055 (652786) 652786..654318 - 1533 WP_001350525.1 protein YeeR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T194133 WP_000854814.1 NZ_CP070482:c651528-651154 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T194133 NZ_CP093369:603270-603443 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT194133 WP_001285584.1 NZ_CP070482:c651985-651617 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT194133 NZ_CP093369:602946-603242 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References