Detailed information of TA system TA194129

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Acetyltransf_4(toxin)
• Location: 4957867..4959375
• Replicon: chromosome
• Accession: NZ_CP070471
• Organism: Pseudomonas aeruginosa strain B17932

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: JT750_RS22695
• Protein ID: WP_074198311.1
• Coordinates: 4958788..4959375 (-)
• Length: 196 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A1C7C4U7
• Locus tag: JT750_RS22690
• Protein ID: WP_003091607.1
• Coordinates: 4957867..4958721 (-)
• Length: 285 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JT750_RS22675	4953286..4953495	+	210	WP_003091604.1	cold-shock protein	-
JT750_RS22680	4953556..4955430	-	1875	WP_003117607.1	MFS transporter	-
JT750_RS22685	4955605..4957770	-	2166	WP_033990934.1	TonB-dependent receptor family protein	-
JT750_RS22690	4957867..4958721	-	855	WP_003091607.1	AraC family transcriptional regulator	Antitoxin
JT750_RS22695	4958788..4959375	-	588	WP_074198311.1	GNAT family N-acetyltransferase	Toxin
JT750_RS22700	4959641..4963120	+	3480	WP_003091610.1	hybrid sensor histidine kinase/response regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (20-152)

Antitoxin

• AraC-type arabinose-binding/dimerisation domain (19-151)

Sequences

Toxin

Protein

• Length: 196 a.a.
• Molecular weight: 21931.26 Da
• Isoelectric Point: 9.8676

>T194129 WP_074198311.1 NZ_CP070471:c4959375-4958788 [Pseudomonas aeruginosa]
MSRRAPMFKPLPITLQRGALRLEPLVEADIPELVSLAEANREALQYMDGPTRPDWYRQSLAEQREGRALPLAVRLGVQLV
GTTRFAEFLPALPACEIGWTWLDQAQHGSGLNRMIKYLMLKHAFDNLRMVRVQLSTAASNLRAQGAIDKLGAQREGVLRN
HRRLAGGRLDDTFVYSITDHEWPQVKATLEASFTG

Nucleotide

• Length: 588 bp

>T194129 NZ_CP093368:4015944-4016321 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACAATGA

Antitoxin

Protein

• Length: 285 a.a.
• Molecular weight: 31895.20 Da
• Isoelectric Point: 7.5295

>AT194129 WP_003091607.1 NZ_CP070471:c4958721-4957867 [Pseudomonas aeruginosa]
VPDETSGEHTHFWRAAELGGVELLHARYVEQRFAPHVHSSFALVIIEQGAQRFRHRGGEHLAPLGSMVLINPDEVHTGSK
AHDAGWLYRGYYPEMEQIDGILDELEIHHGGVPRFADSVIHDQPLARAFVHLHRLLAEHAGSLETQTRWRETMLALFRRH
ARLPEAPKAGQEPLAVARARELLAARLLDPPSLEELAAAVNLSPFHFARVFRRATGMPPHAWLRQRRLEQARALLKSGRA
PLEVALELGFADQSHLGRQFKQAYGVAPGEYRSACARSSLTTYE

Nucleotide

• Length: 855 bp

>AT194129 NZ_CP093368:4015520-4015897 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCATCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAATCACCGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A1C7C4U7