Detailed information of TA system TA194128

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3207785..3209005
• Replicon: chromosome
• Accession: NZ_CP070471
• Organism: Pseudomonas aeruginosa strain B17932

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A3S3XIW1
• Locus tag: JT750_RS14890
• Protein ID: WP_033991668.1
• Coordinates: 3207785..3208363 (-)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JT750_RS14895
• Protein ID: WP_003088112.1
• Coordinates: 3208451..3209005 (-)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JT750_RS14875	3203060..3204337	+	1278	WP_003088104.1	TolC family protein	-
JT750_RS14880	3204327..3206498	+	2172	WP_023106617.1	type I secretion system permease/ATPase	-
JT750_RS14885	3206488..3207675	+	1188	WP_003132729.1	HlyD family efflux transporter periplasmic adaptor subunit	-
JT750_RS14890	3207785..3208363	-	579	WP_033991668.1	HD domain-containing protein	Toxin
JT750_RS14895	3208451..3209005	-	555	WP_003088112.1	helix-turn-helix domain-containing protein	Antitoxin
JT750_RS14900	3209163..3211358	-	2196	WP_003139882.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
JT750_RS14905	3211363..3211824	-	462	WP_003102736.1	(2Fe-2S)-binding protein	-
JT750_RS14910	3212155..3212478	+	324	WP_003102734.1	multidrug efflux SMR transporter	-
JT750_RS14915	3212491..3212889	+	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
JT750_RS14920	3212886..3213437	-	552	WP_003132721.1	XRE family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21947.77 Da
• Isoelectric Point: 5.3655

>T194128 WP_033991668.1 NZ_CP070471:c3208363-3207785 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDTPLRDRLLALWDEYERGETAEALAVKALDKLETLFQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T194128 NZ_CP093368:c3895419-3895012 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT194128 WP_003088112.1 NZ_CP070471:c3209005-3208451 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT194128 NZ_CP093368:c3895666-3895400 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A3S3XIW1