Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4634725..4635535 Replicon chromosome
Accession NZ_CP070464
Organism Klebsiella pneumoniae strain B28906

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag JT748_RS22360 Protein ID WP_004178461.1
Coordinates 4634725..4635258 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag JT748_RS22365 Protein ID WP_002887278.1
Coordinates 4635269..4635535 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JT748_RS22355 4633556..4634677 + 1122 WP_002887282.1 cupin domain-containing protein -
JT748_RS22360 4634725..4635258 - 534 WP_004178461.1 GNAT family N-acetyltransferase Toxin
JT748_RS22365 4635269..4635535 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
JT748_RS22370 4635638..4637071 - 1434 WP_004894565.1 Cu(+)/Ag(+) sensor histidine kinase -
JT748_RS22375 4637061..4637744 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
JT748_RS22380 4637916..4639301 + 1386 WP_004894567.1 efflux transporter outer membrane subunit -
JT748_RS22385 4639319..4639663 + 345 WP_134920081.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T194117 WP_004178461.1 NZ_CP070464:c4635258-4634725 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T194117 NZ_CP093368:c2149231-2149124 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT194117 WP_002887278.1 NZ_CP070464:c4635535-4635269 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT194117 NZ_CP093368:2149278-2149344 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References