Detailed information of TA system TA194114

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2692010..2693598
• Replicon: chromosome
• Accession: NZ_CP070464
• Organism: Klebsiella pneumoniae strain B28906

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A9J6RYF8
• Locus tag: JT748_RS13110
• Protein ID: WP_004892016.1
• Coordinates: 2692010..2693332 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: JT748_RS13115
• Protein ID: WP_004176198.1
• Coordinates: 2693332..2693598 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JT748_RS13095	2687066..2689567	+	2502	WP_004892009.1	fimbrial biogenesis outer membrane usher protein	-
JT748_RS13100	2689555..2690559	+	1005	WP_004892012.1	type 1 fimbrial protein	-
JT748_RS13105	2690701..2691993	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
JT748_RS13110	2692010..2693332	-	1323	WP_004892016.1	type II toxin-antitoxin system HipA family toxin	Toxin
JT748_RS13115	2693332..2693598	-	267	WP_004176198.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
JT748_RS13120	2693863..2694189	-	327	WP_004892019.1	hydroxyisourate hydrolase	-
JT748_RS13125	2694186..2694686	-	501	WP_004892023.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
JT748_RS13130	2694664..2696043	-	1380	WP_004176204.1	purine permease	-
JT748_RS13135	2696388..2697542	+	1155	WP_004892025.1	FAD-dependent urate hydroxylase HpxO	-
JT748_RS13140	2697523..2698446	-	924	WP_004892028.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	mgtC / iroE	2503367..2729080	225713

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49141.44 Da
• Isoelectric Point: 7.5357

>T194114 WP_004892016.1 NZ_CP070464:c2693332-2692010 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T194114 NZ_CP093368:c1371545-1371327 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.36 Da
• Isoelectric Point: 8.5912

>AT194114 WP_004176198.1 NZ_CP070464:c2693598-2693332 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT194114 NZ_CP093368:c1371945-1371571 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG