Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4663827..4664637 Replicon chromosome
Accession NZ_CP070459
Organism Klebsiella pneumoniae strain B28484

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag JT747_RS22840 Protein ID WP_004178461.1
Coordinates 4663827..4664360 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag JT747_RS22845 Protein ID WP_002887278.1
Coordinates 4664371..4664637 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JT747_RS22835 4662658..4663779 + 1122 WP_004214138.1 cupin domain-containing protein -
JT747_RS22840 4663827..4664360 - 534 WP_004178461.1 GNAT family N-acetyltransferase Toxin
JT747_RS22845 4664371..4664637 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
JT747_RS22850 4664740..4666173 - 1434 WP_064162551.1 Cu(+)/Ag(+) sensor histidine kinase -
JT747_RS22855 4666163..4666846 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
JT747_RS22860 4667018..4668403 + 1386 WP_064162550.1 efflux transporter outer membrane subunit -
JT747_RS22865 4668421..4668765 + 345 WP_064162549.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T194099 WP_004178461.1 NZ_CP070459:c4664360-4663827 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T194099 NZ_CP093358:1305850-1306269 [Pseudomonas aeruginosa]
ATGATTGTCTTGGATACGAATGTCGTCTCCGAGGCGATGAAGCCCGAACCCAACCCGGCCGTGCGAGCTTGGTTGAATGA
GCAGGTAGTGGAAACCCTCTACCTGTCCAGCGTGACGCTGGCCGAGCTGCTGTTCGGCATCGGCGCGCTTCCGAACGGGA
AACGTAAGAAAGGCCTGGGCGAAGCCCTGGACGGCTTGCTCGAGCTGTTCGGCGAGCGGGTGCTGATGTTCGACACCGAA
GCAGCTCGCCATTATGCCGAGCTTGCGGTGAAGGCCCGCACAGCCGGAAAGGGTTTCCCAACACCCGACGGATATATTGG
GGCTATTGCGGCCTCCAAGGGATTTATCGTAGCAACACGCGACACCAGCCCTTTTGAAGCGGCTGGGCTTACTGTTATCA
ATCCTTGGAACCATCAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT194099 WP_002887278.1 NZ_CP070459:c4664637-4664371 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT194099 NZ_CP093358:1305599-1305853 [Pseudomonas aeruginosa]
ATGGCAATCCTGACAGTGCGGAATGTGCCGGACGAGGTGCATCGCGCTCTGCGCTTACGGGCCGCCGAGCATGGCCGCAG
TACAGAGGCGGAGGTGCGCGAGATTCTGGAAAGTGCAGTGAAGTCGGAAAAGCGCATCCGCATGGGCGACGCGTTGGCGG
AGCTTGGCCGCCGCGTAGGGCTGACAAACGACGACTTCGCAGTATTGGACCAGGTGCGCGACAAGGTGCCGGCAGAACCG
ATGAGGTTTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References