Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4661859..4662669 Replicon chromosome
Accession NZ_CP070449
Organism Klebsiella pneumoniae strain B18064

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag JT745_RS22830 Protein ID WP_004178461.1
Coordinates 4661859..4662392 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag JT745_RS22835 Protein ID WP_002887278.1
Coordinates 4662403..4662669 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JT745_RS22825 4660690..4661811 + 1122 WP_004214138.1 cupin domain-containing protein -
JT745_RS22830 4661859..4662392 - 534 WP_004178461.1 GNAT family N-acetyltransferase Toxin
JT745_RS22835 4662403..4662669 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
JT745_RS22840 4662772..4664205 - 1434 WP_064162551.1 Cu(+)/Ag(+) sensor histidine kinase -
JT745_RS22845 4664195..4664878 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
JT745_RS22850 4665050..4666435 + 1386 WP_064162550.1 efflux transporter outer membrane subunit -
JT745_RS22855 4666453..4666797 + 345 WP_064162549.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T194067 WP_004178461.1 NZ_CP070449:c4662392-4661859 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T194067 NZ_CP093337:2734684-2735946 [Shinella sp. H4-D48]
ATGAGCCGCGTGCTGGACGTCTTCCTCAAAGACCGCAAGGCCGGCGAGCTCAAGCAGGACAGCGACGCCTCGCTCACCTT
CGCTTACGACGGCGACTACCTCGCCAGTGATCCAATCGCTTTGTCAGTCTCCCTACCGGTGAGGGAAGAACCTTTTGCCG
ATCGCGTCGCACGTCCATTCTTCTCCGGTCTCCTGCCTGATGAAGGCGCTCGACGTCGATTGGCTGCGGCACTGGGCATA
TCCTCGGGTAACGCGTTCGGGCTGCTCGAGGTTATCGGCGGGGAATGTGCGGGCGCCTTGTCGCTTGTTCCGCCAGGACA
GTTCCCACCGGCCTCGACGACAGACGATGCCGAGGCACTCGATGAGCAGCTCCTCGAAGGGATCCTCTCTTTGCTCAGAC
AGAGGCCCCTGCTCGGCGGAGAAGGTGACGTGCGCCTTTCGCTTGCCGGCGCCCAGGACAAGCTGGCCGTGACCGTCATC
AACGGCCACATATTGCTGCCCCGGGGCGGGCGGCCGACGACGCACATCCTCAAACCCTTCATCGAGGGCCTTGATGGCAC
GGTGGAGAACGAAGTGTTCTGCATGAGCCTGGGACGCCGTCTCGGCCTCGATGTCCCCGCTGTGTCGAAGGGCACGGCTG
GCAAGGTCGATTACTTCCTGGTCGAGCGCTACGATCGCCTGACGCTTGAAGACGGGCGGATCGAGCGGCTGCACCAGGAA
GACTTCTGCCAGGCGCTGAGCGTGCCCCCCGAGCTCAAGTATGAGGAAGAAGGAGGCCCCGGCATCAGTCAGTGCCTCCA
GCTGATCCGGCAAACGACGGCCAAGCCCGCGGCCGAGACATTGCGCTTCCAGCGAATGCTGATGTTCCACTACCTGATTG
GCAATGCTGACGCCCATGCCAAGAACTACGCGCTGCTCTATCGTGGGAAAGCGCCGGACCTGGCACCGATGTATGATGCC
ATCTGCACGGCTGCCTATCCACGACTGTCAAAGAAGATGGCGATGGCACTCGGCGGCAGGTCACTACCAGACACGATCCA
GATGGAGCACTGGGCATCGCTCGTGCCGTCCAACAAGGCCGCGACGCGCATGCTGGCCGGCGAGTTGGTCCGCATGGCGG
ACGCGATCGGGCTGGAAGCCGACGGGCTGATGGACGAGATGTCCGAGAACGGCACCTACCATCCGATATTAAAGACCGTC
CGGAAAATAATCGACACCAGATGTGCCCTGATGCGAAGGGCGGTGGAAAAGCCGAGCTTGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT194067 WP_002887278.1 NZ_CP070449:c4662669-4662403 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT194067 NZ_CP093337:2734463-2734687 [Shinella sp. H4-D48]
ATGGCGACGGTCTCTGATAGCGTAGCACTCGGCCAGCTGATCCGCGCCGAGCGAAAACGTCAGCAACTGACTCAGGAACA
ACTTGCCGGCGTCGCAGGCGTGGGCGTGCGGTTCGTGCGCGAGCTGGAGAGCGGCAAGGAAAGCTGTCGGGTCGGACTTG
CTCTGACCGTCCTGCAGACGCTTGGCTTGACCGTGAGCATCACCTCTCGCGGTGCCTCCTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References