Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5270521..5271146 Replicon chromosome
Accession NZ_CP070445
Organism Klebsiella pneumoniae strain B12097

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag JT744_RS25645 Protein ID WP_002882817.1
Coordinates 5270521..5270904 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag JT744_RS25650 Protein ID WP_004150355.1
Coordinates 5270904..5271146 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JT744_RS25630 5267887..5268789 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
JT744_RS25635 5268786..5269421 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
JT744_RS25640 5269418..5270347 + 930 WP_064162495.1 formate dehydrogenase accessory protein FdhE -
JT744_RS25645 5270521..5270904 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
JT744_RS25650 5270904..5271146 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
JT744_RS25655 5271351..5272268 + 918 WP_004181612.1 alpha/beta hydrolase -
JT744_RS25660 5272282..5273223 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
JT744_RS25665 5273268..5273705 - 438 WP_064162494.1 D-tyrosyl-tRNA(Tyr) deacylase -
JT744_RS25670 5273702..5274562 - 861 WP_004146232.1 virulence factor BrkB family protein -
JT744_RS25675 5274556..5275155 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T194053 WP_002882817.1 NZ_CP070445:c5270904-5270521 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T194053 NZ_CP093334:c270502-269171 [Raoultella planticola]
ATGGCTACTCTCGTGACATGGATGAACAATGAGCGAGTCGGCGAACTTATCAAGCAGGCGAACGGCGCCCATCTCTTTCG
CTATGAAGAGGCGTGGATGCGCAATCCTCGCGCCAGGCCGCTCTCTCTGTCGCTCCCGCTGCAGTACGGTAACATCACCG
ACGATGCGGTGTTTAACTATTTTGACAACCTGCTGCCGGACAGCCCGCGGGTGCGGGACCGCATTGTTAAGCGCTATCAG
GCGCGTTCAAAGCAGCCTTTCGATCTGCTGGCCGAGATCGGACGCGACAGCGTCGGGGCGGTCACGTTACTCCCGCCAGG
GGAAAACGCAACCCGGAGAGCGTTGAGCTGGGAGGCGCTGGATAGCCACGCCCTGGATGCGCTGCTGACGGCCTACCAGT
CGGATATACCGCTCGGGATGGTCAACGAACACGATGATTTCAGGATCTCGGTGGCCGGAGCCCAGGAGAAAACCGCGCTG
CTGAAAATCGGCGAGCAGTGGTGTATACCGACGGGGACCACGCCGACCACCCATATCATTAAACTGCCTATCGGCGAGAT
CAAACAGCCGGATGCGGTGCTGGATCTCAGCGAAAGCGTAGATAACGAATATTTGTGTCTGGCGCTGGCCCGGGCGCTGG
GTTTTGCCGTTCCGCGCGCCAGCATTATCCAGACCGCCAGCATCAGGGCGCTGGCGGTTGAGCGTTTCGACCGTCGTTGG
GCGCAGGAGAAGACGGTGCTGCTGCGTTTACCGCAGGAAGATCTGTGTCAGGCCTTCGGCATTCCGTCATCGATTAAGTA
TGAGTCCGACGGCGGGCCGGGCATTCGGGCGATTATGGCTTTTCTGGTTGGATCCAGCGAGGCGCTCGAGGATCGCTATA
ACTTTATGAAGTTTATGGTGTTCCAGTGGCTGATTGGCGCAACCGATGGCCACGCGAAAAACTTCTCGATCTTCCTCCAG
CCGGGCGGCAGCTATCGCTTAACCCCATTCTACGACATTATCTCCGCGTTTCCGCTGCTTGGCGGGAAGGGGCTGCACCT
GAGCGATCTCCGGCTGTCAATGAGCCTGAAGGCCAGTAAAGGGCGCAAAACGGAGATCCAGACGCTCTATCCGCGTCACT
TCCTCGCCACGGCGAAAGAGGTGGGGTTTGCCGAGGAGCAGATGCTGGAGATTCTGCGCTATTTTGTCGATAACGTTCCC
CACGCGGTTGCTGCGGTAAAGGAAACTTTACCGCATGACTTTTCACCACCGGTGTATGAGGCGATCACCGATCGCTTACT
GTGGGTCCATTCACGCTTGCAGAACGCAATGGCGGCGTTACCGTCGGGCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT194053 WP_004150355.1 NZ_CP070445:c5271146-5270904 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT194053 NZ_CP093334:c270774-270502 [Raoultella planticola]
ATGAGCCCTTCACCAGGAATATACAGCCCGGTTCAGCTGGCAAACTATCTCAAGCTGGTGCGCCAGAAAAACCAGTGGAC
GCAGAGCGATCTGGCTCAGCGTATGGGGATTAAACAGGCTACCGTCTCTAACTTTGAAAATCATCCCGAGAAAACGACGT
TAACCACGGTGTTTAAAATTTTACAGTCACTGGAACTGTCGATGGCGATTTGCGAAAAAAATGCGCCGGATACCAACGAT
AGCAGTGATAACCCACAGGATCTGGACTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References