Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 107722..108245 Replicon plasmid p3B5617
Accession NZ_CP070438
Organism Klebsiella pneumoniae strain B5617

Toxin (Protein)


Gene name ccdB Uniprot ID V5RNQ6
Locus tag JT733_RS28370 Protein ID WP_015632464.1
Coordinates 107722..108027 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V5RQR8
Locus tag JT733_RS28375 Protein ID WP_015632465.1
Coordinates 108027..108245 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JT733_RS28325 102752..103180 - 429 WP_015632456.1 plasmid partitioning/stability family protein -
JT733_RS28330 103173..104153 - 981 WP_015632457.1 partitioning protein -
JT733_RS28335 104514..104696 + 183 WP_015632458.1 type II toxin-antitoxin system HicA family toxin -
JT733_RS28340 104722..105165 + 444 WP_015632459.1 type II toxin-antitoxin system HicB family antitoxin -
JT733_RS28345 105371..105493 - 123 Protein_112 transcriptional regulator -
JT733_RS28350 105507..105734 - 228 WP_016162072.1 hypothetical protein -
JT733_RS28355 106164..106421 + 258 WP_016162071.1 helix-turn-helix domain-containing protein -
JT733_RS28360 106625..107191 - 567 WP_015632462.1 hypothetical protein -
JT733_RS28365 107233..107577 - 345 WP_015632463.1 hypothetical protein -
JT733_RS28370 107722..108027 - 306 WP_015632464.1 type II toxin-antitoxin system toxin CcdB Toxin
JT733_RS28375 108027..108245 - 219 WP_015632465.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
JT733_RS28380 108431..109456 + 1026 WP_015632466.1 IS110 family transposase -
JT733_RS28385 109751..110236 + 486 Protein_120 IS5 family transposase -
JT733_RS28390 110400..110831 + 432 WP_015632467.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
JT733_RS28395 110831..112102 + 1272 WP_016162068.1 Y-family DNA polymerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1A / blaOXA-9 / ant(3'')-Ia / aac(6')-Ib / blaCTX-M-15 / sul1 / qacE / dfrA1 / ARR-3 - 1..112495 112495
- flank IS/Tn - - 109751..110278 527


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11361.90 Da        Isoelectric Point: 5.5252

>T194021 WP_015632464.1 NZ_CP070438:c108027-107722 [Klebsiella pneumoniae]
MQFTVYEYTRESHYRLFVDVQSDIVETPGRRIVIPLLESHHLSEKVNQQLFPVVSVNGKEYRAVTSELSGVSVSVIGDAV
ADLSHEANAIKNALNLMFWGI

Download         Length: 306 bp

>T194021 NZ_CP093323:c3485812-3485716 [Serratia plymuthica]
AACAAGCCCTGCACAAAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTAGACCGAATATAGGAATC
GTATTCGGTCTTTTTTT

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8194.13 Da        Isoelectric Point: 6.2224

>AT194021 WP_015632465.1 NZ_CP070438:c108245-108027 [Klebsiella pneumoniae]
MKHRASVTVDKEHYLELLAAGVNISGLVNEAIGREARRIQAERWKAENREGMEEVANFIAQHGSFADENRSW

Download         Length: 219 bp

>AT194021 NZ_CP093323:3485716-3485856 [Serratia plymuthica]
AAAAAAAGACCGAATACGATTCCTATATTCGGTCTAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCAT
TTTGTGCAGGGCTTGTTCAGCCATGCACTTTAAGAGTAGCTTACCGCGCTAGTTTTGCCAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0ND19

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0L1T7

References