Detailed information of TA system TA194020

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 6174..6817
• Replicon: plasmid p3B5617
• Accession: NZ_CP070438
• Organism: Klebsiella pneumoniae strain B5617

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: JT733_RS27835
• Protein ID: WP_071854281.1
• Coordinates: 6401..6817 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: JT733_RS27830
• Protein ID: WP_001261282.1
• Coordinates: 6174..6404 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JT733_RS27790	1519..2145	+	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-
JT733_RS27795	2265..2444	+	180	WP_006788217.1	hypothetical protein	-
JT733_RS27800	2876..3670	-	795	WP_205450173.1	tyrosine-type recombinase/integrase	-
JT733_RS27805	3663..3824	-	162	WP_032495756.1	hypothetical protein	-
JT733_RS27810	3868..4872	-	1005	WP_016162067.1	hypothetical protein	-
JT733_RS27815	4937..5248	-	312	WP_024191724.1	hypothetical protein	-
JT733_RS27820	5297..5611	-	315	WP_015632378.1	hypothetical protein	-
JT733_RS27825	5756..6217	-	462	WP_165763053.1	hypothetical protein	-
JT733_RS27830	6174..6404	+	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JT733_RS27835	6401..6817	+	417	WP_071854281.1	type II toxin-antitoxin system VapC family toxin	Toxin
JT733_RS27840	6891..7127	+	237	WP_001348075.1	AAA family ATPase	-
JT733_RS27845	7174..7878	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
JT733_RS27850	8100..9098	-	999	WP_001240354.1	hypothetical protein	-
JT733_RS27855	9328..10025	+	698	Protein_14	IS1 family transposase	-
JT733_RS27860	10172..10774	+	603	WP_086538008.1	DUF1819 family protein	-
JT733_RS27865	10771..11373	+	603	WP_000566901.1	DUF1788 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1A / blaOXA-9 / ant(3'')-Ia / aac(6')-Ib / blaCTX-M-15 / sul1 / qacE / dfrA1 / ARR-3	-	1..112495	112495

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15086.51 Da
• Isoelectric Point: 7.1084

>T194020 WP_071854281.1 NZ_CP070438:6401-6817 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T194020 NZ_CP093323:c2311912-2311730 [Serratia plymuthica]
GTGAAAAGTTCAGAACTGATCAGGCTGTTAGAACGAGAAGGTTGGGTTTTAGAGCGGATAAAGGGAAGCCACCATCAGTT
TAAACACCCGGGATCTCGTTTGGTGATCACCGTTCCACACCCGCGTAAAGATCTGAAGCCGGGAACGCTGCGCCAGATCA
TGAAAGATGCCGGGCTGTGCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT194020 WP_001261282.1 NZ_CP070438:6174-6404 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT194020 NZ_CP093323:c2311700-2311287 [Serratia plymuthica]
ATGTTCTACCCTGCTTATGTACATTCAGACCTCGACGGCAGCGCCAGCGGTTTCTTCCCCGATGTGCCGGGCTGTTATTT
TGCCGGCGATACCCTCGATAAAGCCTTTGAAGATGCCAAAGGCGCGCTGGATGCGCATTTCGAACTTCTGAGCGAAGACA
ACCAGGCCATTCCGCGCCCACAGGCCGTTTCCTTCCATCTGGCACAGGAAAACGACACCCTGAACGGCGGGCAGTGGCTG
CTGGTCGATATCAATATGGATAAGTTCGATGGGCGGGCGGAACGTATCAATATCACCCTGCCGCACCGCTTGCTGAACCG
CATCGATTCGCTGGTGCAGCAGCATCCTGGCTATGGGAGCCGCAGCGCCTTTTTGGCCGCCGCCGCCCGTAATGAATTAT
TGAAAGTCGGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3