Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 112867..113603 Replicon plasmid p1B5617
Accession NZ_CP070436
Organism Klebsiella pneumoniae strain B5617

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag JT733_RS26800 Protein ID WP_003026803.1
Coordinates 113121..113603 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag JT733_RS26795 Protein ID WP_003026799.1
Coordinates 112867..113133 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JT733_RS26750 108929..109291 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
JT733_RS26755 109341..109691 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
JT733_RS26760 110049..110318 + 270 WP_004152102.1 hypothetical protein -
JT733_RS26765 110306..110881 + 576 WP_004152103.1 hypothetical protein -
JT733_RS26770 110912..111406 + 495 WP_004152104.1 DNA-binding protein -
JT733_RS26775 111450..111818 + 369 WP_004152105.1 hypothetical protein -
JT733_RS26780 111852..112055 + 204 WP_004152106.1 hemolysin expression modulator Hha -
JT733_RS26785 112104..112361 + 258 WP_004152107.1 hypothetical protein -
JT733_RS26790 112437..112691 + 255 WP_004152108.1 hypothetical protein -
JT733_RS26795 112867..113133 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
JT733_RS26800 113121..113603 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
JT733_RS26805 113804..115207 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
JT733_RS26810 115236..115868 - 633 WP_001567369.1 hypothetical protein -
JT733_RS26815 116098..117444 + 1347 WP_020314316.1 ISNCY family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr - 1..151424 151424


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T194019 WP_003026803.1 NZ_CP070436:113121-113603 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T194019 NZ_CP093323:1503044-1503313 [Serratia plymuthica]
ATGCGGATAGAATGGAGTCGTGAAGCTCAGGAAGAGCTATGGAACATTATTGATTATATTGATGATAGAAACCCGCAGGC
GGCGCTCATTTTACAGCGCGAGATCGAAACGGCGGTGCATTCGCTGTCGCTGAATCCCTTTATGTACCGGTTGGGCAGAG
TAGATAACACTCGTGAGATTGTCGTCCATCCCAACTACCTGATTGTTTATAGAATCATCGGACATATAGAAGTTCTTGGC
GTGATTCACGCTAAACGAGAATATCCCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT194019 WP_003026799.1 NZ_CP070436:112867-113133 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT194019 NZ_CP093323:1502846-1503040 [Serratia plymuthica]
ATGGTGATCAAAACCTCCCTCTCCACCCAGCCCACAGCTGAGGTAGCCGAACAAGGCGAAAGTTACGATCGTTGGTTCCG
GGCCAAGATTGAAGCAGCCATTAACAGCGAAAAGCCAAGAATTCCGCACGAGCAGGTCATGAACGAGATGCAAATTCGTT
TAGCGGCGAAGAAAAAGATACGCAAAATGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References