Detailed information of TA system TA194018

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 38340..38983
• Replicon: plasmid p1B5617
• Accession: NZ_CP070436
• Organism: Klebsiella pneumoniae strain B5617

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: JT733_RS26410
• Protein ID: WP_014386165.1
• Coordinates: 38567..38983 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: JT733_RS26405
• Protein ID: WP_032408893.1
• Coordinates: 38340..38570 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JT733_RS26380	33413..34408	-	996	WP_000611945.1	NAD(P)H-quinone oxidoreductase	-
JT733_RS26385	34415..35563	-	1149	WP_014386168.1	ABC transporter ATP-binding protein	-
JT733_RS26390	35734..36891	-	1158	WP_032408895.1	acetylornithine deacetylase	-
JT733_RS26395	36907..37581	-	675	WP_014386166.1	DUF1028 domain-containing protein	-
JT733_RS26400	37586..38020	-	435	WP_000103648.1	RidA family protein	-
JT733_RS26405	38340..38570	+	231	WP_032408893.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JT733_RS26410	38567..38983	+	417	WP_014386165.1	PIN domain-containing protein	Toxin
JT733_RS26415	39306..40274	+	969	WP_004099053.1	IS5-like element IS903B family transposase	-
JT733_RS26420	40454..40828	-	375	WP_032408891.1	hypothetical protein	-
JT733_RS26425	40884..41210	-	327	WP_004152639.1	hypothetical protein	-
JT733_RS26430	41207..41935	-	729	WP_032420965.1	plasmid SOS inhibition protein A	-
JT733_RS26435	41932..42363	-	432	WP_001568057.1	conjugation system SOS inhibitor PsiB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr	-	1..151424	151424
	-	flank	IS/Tn	-	-	39306..40274	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-130)

Antitoxin

• SpoVT-AbrB domain (7-55)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15127.44 Da
• Isoelectric Point: 7.8841

>T194018 WP_014386165.1 NZ_CP070436:38567-38983 [Klebsiella pneumoniae]
MTKTYMLDTNICSYIMREQPEAVLKRLEQAVLRRHRIVVSAITYAEMRFGAIGKRASPRHVQLVDAFCARLDAILAWDRA
AVDATTEVKATLAAAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVAGLTFEDWVQ

Nucleotide

• Length: 417 bp

>T194018 NZ_CP093323:1457414-1457803 [Serratia plymuthica]
ATGTATATGTTTGACACCAATACCGTGAGCCACCTGTTTCGCCGGCACCCGGGTTTGCTGAGTGCGATGGAAAAGGTGCC
GCCTTCCACCGTATGCATTTCCAGCATCACGGAGGCAGAGCTGCTTTTTGGCGTGGCCAAGCGGCAGAGTAAAGCGTTGA
AAGCCATGGTAACGGCGTTTTTAGCCGCGGTGACCGTTTATGACTGGGATAGCGAAGCTGCCCGCTGTTATGGCGTGATG
CGGGCGAGCATGGAGAAAAAAGGCAAAGTCCTGGGCGCGCTGGACCAGTTGATTGCCGCGCATGCGCTTAGCCGGGGCGC
GACAATGGTCACTAGCGATCGGGCCTTCGGCATGGTGTCCGGGCTGGACGTTGAAGACTGGACCCTATAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8854.89 Da
• Isoelectric Point: 4.5112

>AT194018 WP_032408893.1 NZ_CP070436:38340-38570 [Klebsiella pneumoniae]
MRTVSVFRNGNNRAIRLPRDLDFDGVNELEIIREGDTLILRPVKPSWSSFRQEEKADADFMAERENVVSDEGRFEL

Nucleotide

• Length: 231 bp

>AT194018 NZ_CP093323:1457148-1457411 [Serratia plymuthica]
ATGGAAAGAATCGCAAAGCTGTTTAAAAATGGCAGAAATCAGGCGGTGAGGTTACCCGTGGAATTCGAATTTGACACAGA
TCGCGTATATATCCGCCGCGATCGGGAGGGGAATGTGATTTTATCGAAATACCCTGCAAAACCGGACAGTTGGGAGCCGC
TGTTGAACATAATCGCCAAAACGCCGGTGCCGCATGATTTTCTGGGCGCGGATGAACGCCAACAGGGCATTGCCGACCGG
GATCCTTTCGCAGAGGAAGAGTAA