Detailed information of TA system TA194016

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5243305..5243930
• Replicon: chromosome
• Accession: NZ_CP070435
• Organism: Klebsiella pneumoniae strain B5617

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JT733_RS25785
• Protein ID: WP_040182550.1
• Coordinates: 5243305..5243688 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J2DFR0
• Locus tag: JT733_RS25790
• Protein ID: WP_004150355.1
• Coordinates: 5243688..5243930 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JT733_RS25770	5240671..5241573	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
JT733_RS25775	5241570..5242205	+	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
JT733_RS25780	5242202..5243131	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
JT733_RS25785	5243305..5243688	-	384	WP_040182550.1	type II toxin-antitoxin system VapC family toxin	Toxin
JT733_RS25790	5243688..5243930	-	243	WP_004150355.1	ribbon-helix-helix domain-containing protein	Antitoxin
JT733_RS25795	5244135..5245052	+	918	WP_023302328.1	alpha/beta hydrolase	-
JT733_RS25800	5245066..5246007	-	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
JT733_RS25805	5246052..5246489	-	438	WP_002882809.1	D-tyrosyl-tRNA(Tyr) deacylase	-
JT733_RS25810	5246486..5247346	-	861	WP_004146232.1	virulence factor BrkB family protein	-
JT733_RS25815	5247340..5247939	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14376.67 Da
• Isoelectric Point: 9.5352

>T194016 WP_040182550.1 NZ_CP070435:c5243688-5243305 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLKLITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T194016 NZ_CP093323:1046918-1047340 [Serratia plymuthica]
GTGGCCCAGTGGCGATGTAATGTCCGCATTTCCTGGCGCACCCAGTTACTTTCGCTGCTGACCCACGGCGTTCTGATCCT
GCTGATCCTGATTTCGCCCTGGCCGGAAGGCTACGGCCCGATCTGGCTGGTGTTGTTGACGCTGGTGGTATTTGAATGCA
TCCGCAGCCAAAAGCGCATTGCGTCGCGGCAGGGCGAACTGCGTCTGCTGGCGAATAAGCGGTTAAGTTGGCACGGACAG
GAATGGTTGCTGGCAAAGCAACCCTGGATGCCGCGTTACGGCATTTTGCTGACGCTGCAGCCGACGCAGGGCAAAAAACG
CCGCCGGCTGTGGCTGGCCTCCGACAGCATGGGCAAAGCCGAATGGCGGCATCTGCGCCAGTTGCTGCTGCACCCGTCGG
CCGATGATGACGAGGAACCCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9567.83 Da
• Isoelectric Point: 4.3509

>AT194016 WP_004150355.1 NZ_CP070435:c5243930-5243688 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT194016 NZ_CP093323:1046671-1046937 [Serratia plymuthica]
ATGGATATTAATAATAAAGCACGTATTCACTGGGCCTGCCGCCGTGGCATGCGTGAGCTGGACATCTCAATCATGCCGTT
CTTCGAGCATGAGTACGACCAGTTGAATGACACAGACAAAGCGTTGTTTATCCGCCTGCTTGAGAGCGACGATCCCGATC
TGTTCAACTGGTTAATGAATCATGGCGCTCCGCAAGACAGTGAACTGCAGAGAATGGTGACACTGATCCAGACGCGAAAT
AAAGACCGTGGCCCAGTGGCGATGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GGU9