Detailed information of TA system TA194015

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4924927..4926608
• Replicon: chromosome
• Accession: NZ_CP070435
• Organism: Klebsiella pneumoniae strain B5617

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: J2XCI1
• Locus tag: JT733_RS24320
• Protein ID: WP_004146663.1
• Coordinates: 4924927..4925844 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A6TH05
• Locus tag: JT733_RS24325
• Protein ID: WP_004177809.1
• Coordinates: 4925850..4926608 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JT733_RS24300	4920233..4921753	+	1521	WP_004210021.1	sugar ABC transporter ATP-binding protein	-
JT733_RS24305	4921750..4922748	+	999	WP_012737175.1	ribose ABC transporter permease	-
JT733_RS24310	4922779..4923720	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
JT733_RS24315	4923795..4924802	+	1008	WP_004178300.1	carbohydrate kinase family protein	-
JT733_RS24320	4924927..4925844	-	918	WP_004146663.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
JT733_RS24325	4925850..4926608	-	759	WP_004177809.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
JT733_RS24330	4926694..4927422	-	729	WP_004177810.1	response regulator transcription factor	-
JT733_RS24335	4927695..4929842	+	2148	WP_012737173.1	formate dehydrogenase H subunit alpha, selenocysteine-containing	-
JT733_RS24340	4930072..4930758	+	687	WP_004151733.1	sel1 repeat family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34659.10 Da
• Isoelectric Point: 5.4707

>T194015 WP_004146663.1 NZ_CP070435:c4925844-4924927 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIAYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T194015 NZ_CP093323:c369322-369083 [Serratia plymuthica]
ATGGCAATTTATGACTACCTGGAAACAAAGAATCCGGCCGCAGCGATAGAAATTGATAGCTTGATTTCAGCAGCTGCGAC
ACGACTCTCACAAAACCCGTCCAGTGGAAAACCAGGCCGAGTACCCGGTACACATGAAATGGTTATTCATCATTCCTATT
TCCTGGTTTATGAATTAATCAACGGGTTAATCATTATGCTGGCCGTAGTGCATGCCCGCCGTCAGTGGCCCGCCAATTAA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28548.92 Da
• Isoelectric Point: 10.0870

>AT194015 WP_004177809.1 NZ_CP070435:c4926608-4925850 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT194015 NZ_CP093323:c369647-369378 [Serratia plymuthica]
ATGTCTGAATCTACCGTGGTTATCCGAGTGGATGAGGAATTAAAAATCGCCTTTGCCAGCGCTGCCAAAGCAGCCGATCG
CACCGCATCTCAGTTGTTGCGCGATTTCATGCGTGATTTTGTCAGTCGGCAAACTCATCAGAAAGAGTATGAGCAGTGGC
TACAGGAAAAAGTGGACTTAAGCCGTAAAGCGCTTAATGAAGGAAAAATCACTGATAATGAAGCCGTTGAGGCCTACTTC
GCTGAGCGCAGGTCAAAACTGACAAGGTAA

Structures

Toxin

• AlphaFold DB: J2XCI1

Antitoxin

• AlphaFold DB: A6TH05