Detailed information of TA system TA194013

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 4680194..4681004
• Replicon: chromosome
• Accession: NZ_CP070435
• Organism: Klebsiella pneumoniae strain B5617

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: A0A332NV98
• Locus tag: JT733_RS23140
• Protein ID: WP_014908042.1
• Coordinates: 4680194..4680727 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: -
• Locus tag: JT733_RS23145
• Protein ID: WP_023343051.1
• Coordinates: 4680738..4681004 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JT733_RS23135	4679025..4680146	+	1122	WP_012737335.1	cupin domain-containing protein	-
JT733_RS23140	4680194..4680727	-	534	WP_014908042.1	GNAT family N-acetyltransferase	Toxin
JT733_RS23145	4680738..4681004	-	267	WP_023343051.1	DUF1778 domain-containing protein	Antitoxin
JT733_RS23150	4681107..4682540	-	1434	WP_023343050.1	Cu(+)/Ag(+) sensor histidine kinase	-
JT733_RS23155	4682530..4683213	-	684	WP_002887273.1	copper response regulator transcription factor CusR	-
JT733_RS23160	4683386..4684771	+	1386	WP_069377349.1	efflux transporter outer membrane subunit	-
JT733_RS23165	4684789..4685133	+	345	WP_004192220.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19795.68 Da
• Isoelectric Point: 5.2614

>T194013 WP_014908042.1 NZ_CP070435:c4680727-4680194 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGLGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T194013 NZ_CP093322:3693276-3693479 [Yersinia intermedia]
ATGACAAAAACTGACTACCTGATGCGTTTAAGAAAATGTACGACTATTGATACATTAGAACGTGTAATTGAAAAGAATAA
GTATGAGCTTTCCGATGATGAGCTGGAATTGTTTTACTCAGCAGCAGACCATCGCCTGGCTGAACTCACAATGAATAAGC
TTTACGATAAAATTCCTCCTACGGTTTGGCAACATGTGAAATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10021.46 Da
• Isoelectric Point: 10.5807

>AT194013 WP_023343051.1 NZ_CP070435:c4681004-4680738 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRRVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT194013 NZ_CP093322:3692860-3693228 [Yersinia intermedia]
ATGGATGAGTACTCGCCTAAACGGCATGACATTGCCCAGTTGAAGTTTCTATGTGAGAGCCTGTATGATGAAGGCATTGC
TACCCTTGGTGACAGCCATCATGGCTGGGTAAATGACCCAACATCTGCCGTAAATCTGCAACTCAATGATTTGATCGAGC
ATATAGCTTCATTTGTGATGAGTTTTAAAATAAAATACCCGGATGACGGCGACCTTTCAGAGTTAGTGGAAGAATATTTA
GACGATACATATACTCTGTTTAGTAGTTACGGAATCAATGATCCTGAATTGAGGCGCTGGCAAAAAACCAAAGAGCGTTT
ATTCAGACTTTTCTCAGGGGAATACACCTGTACCCTGATGAAAACTTAA

Structures

Toxin

• AlphaFold DB: A0A332NV98