Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4057023..4057642 Replicon chromosome
Accession NZ_CP070430
Organism Klebsiella pneumoniae strain B3923

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag JT729_RS19910 Protein ID WP_002892050.1
Coordinates 4057424..4057642 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag JT729_RS19905 Protein ID WP_002892066.1
Coordinates 4057023..4057397 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JT729_RS19895 4052175..4053368 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JT729_RS19900 4053391..4056537 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
JT729_RS19905 4057023..4057397 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
JT729_RS19910 4057424..4057642 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
JT729_RS19915 4057805..4058371 + 567 WP_002892030.1 maltose O-acetyltransferase -
JT729_RS19920 4058343..4058483 - 141 WP_004147370.1 hypothetical protein -
JT729_RS19925 4058504..4058974 + 471 WP_002892026.1 YlaC family protein -
JT729_RS19930 4058949..4060400 - 1452 WP_064162152.1 PLP-dependent aminotransferase family protein -
JT729_RS19935 4060501..4061199 + 699 WP_004177238.1 GNAT family N-acetyltransferase -
JT729_RS19940 4061196..4061336 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
JT729_RS19945 4061336..4061599 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T193996 WP_002892050.1 NZ_CP070430:4057424-4057642 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T193996 NZ_CP093319:c51244-50966 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT193996 WP_002892066.1 NZ_CP070430:4057023-4057397 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT193996 NZ_CP093319:c51513-51244 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAAGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References