Detailed information of TA system TA193994

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2677105..2678693
• Replicon: chromosome
• Accession: NZ_CP070430
• Organism: Klebsiella pneumoniae strain B3923

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: JT729_RS13095
• Protein ID: WP_064162061.1
• Coordinates: 2677105..2678427 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: JT729_RS13100
• Protein ID: WP_002904790.1
• Coordinates: 2678427..2678693 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JT729_RS13080	2672161..2674662	+	2502	WP_064162059.1	fimbrial biogenesis outer membrane usher protein	-
JT729_RS13085	2674650..2675654	+	1005	WP_064162060.1	type 1 fimbrial protein	-
JT729_RS13090	2675796..2677088	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
JT729_RS13095	2677105..2678427	-	1323	WP_064162061.1	type II toxin-antitoxin system HipA family toxin	Toxin
JT729_RS13100	2678427..2678693	-	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
JT729_RS13105	2678959..2679285	-	327	WP_064162062.1	hydroxyisourate hydrolase	-
JT729_RS13110	2679282..2679782	-	501	WP_064162063.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
JT729_RS13115	2679760..2681139	-	1380	WP_040245186.1	purine permease	-
JT729_RS13120	2681485..2682639	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
JT729_RS13125	2682620..2683543	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49155.46 Da
• Isoelectric Point: 7.5357

>T193994 WP_064162061.1 NZ_CP070430:c2678427-2677105 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSTHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T193994 NZ_CP093317:c4245900-4245727 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT193994 WP_002904790.1 NZ_CP070430:c2678693-2678427 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT193994 NZ_CP093317:c4246224-4245928 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0H3GXD8