Detailed information of TA system TA193965

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 84312..84955
• Replicon: plasmid p1B2663
• Accession: NZ_CP070421
• Organism: Klebsiella quasipneumoniae strain B2663

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: JT726_RS26525
• Protein ID: WP_001044770.1
• Coordinates: 84312..84728 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: JT726_RS26530
• Protein ID: WP_001261282.1
• Coordinates: 84725..84955 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JT726_RS26500	79837..80262	+	426	Protein_75	IS1-like element IS1B family transposase	-
JT726_RS26505	80277..81026	+	750	Protein_76	tryptophan--tRNA ligase	-
JT726_RS26510	81179..82576	-	1398	WP_032452481.1	diguanylate cyclase	-
JT726_RS26515	82797..83494	-	698	Protein_78	IS1 family transposase	-
JT726_RS26520	83528..84238	-	711	WP_004152334.1	ATP-binding protein	-
JT726_RS26525	84312..84728	-	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
JT726_RS26530	84725..84955	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JT726_RS26535	84912..85373	+	462	Protein_82	hypothetical protein	-
JT726_RS26540	85543..85761	+	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	-
JT726_RS26545	85763..86068	+	306	WP_001568026.1	type II toxin-antitoxin system toxin CcdB	-
JT726_RS26550	86237..86632	+	396	WP_072158608.1	hypothetical protein	-
JT726_RS26555	86659..86982	+	324	WP_022644730.1	hypothetical protein	-
JT726_RS26560	86979..87995	+	1017	WP_020315256.1	hypothetical protein	-
JT726_RS26565	88148..88852	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
JT726_RS26570	88906..89280	-	375	Protein_89	DUF86 domain-containing protein	-
JT726_RS26575	89277..89567	-	291	WP_001247892.1	nucleotidyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..134892	134892
	-	inside	IScluster/Tn	-	-	70045..92530	22485

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T193965 WP_001044770.1 NZ_CP070421:c84728-84312 [Klebsiella quasipneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T193965 NZ_CP093314:324653-325021 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT193965 WP_001261282.1 NZ_CP070421:c84955-84725 [Klebsiella quasipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT193965 NZ_CP093314:324435-324656 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3