Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 4622959..4623593 Replicon chromosome
Accession NZ_CP070379
Organism Streptomyces tsukubensis strain L20

Toxin (Protein)


Gene name vapC Uniprot ID I2N1L9
Locus tag JVE63_RS18810 Protein ID WP_006348208.1
Coordinates 4622959..4623381 (-) Length 141 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A7G3UFR9
Locus tag JVE63_RS18815 Protein ID WP_040915655.1
Coordinates 4623378..4623593 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JVE63_RS18780 4618400..4619218 + 819 WP_006348202.1 DUF817 domain-containing protein -
JVE63_RS18785 4619308..4619781 + 474 WP_006348203.1 carboxymuconolactone decarboxylase family protein -
JVE63_RS18790 4619923..4620255 - 333 WP_198965075.1 cupin domain-containing protein -
JVE63_RS18795 4620416..4620748 - 333 WP_006348205.1 helix-turn-helix transcriptional regulator -
JVE63_RS18800 4620748..4621044 - 297 WP_078902262.1 type II toxin-antitoxin system RelE/ParE family toxin -
JVE63_RS18805 4621198..4622502 - 1305 WP_006348207.1 cation:proton antiporter -
JVE63_RS18810 4622959..4623381 - 423 WP_006348208.1 PIN domain nuclease Toxin
JVE63_RS18815 4623378..4623593 - 216 WP_040915655.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
JVE63_RS18820 4624037..4624696 + 660 WP_040915657.1 HAD family hydrolase -
JVE63_RS18825 4624677..4625951 - 1275 WP_040915674.1 hypothetical protein -
JVE63_RS18830 4626289..4626648 + 360 WP_006348212.1 hypothetical protein -
JVE63_RS18835 4626744..4627955 + 1212 WP_040915676.1 radical SAM protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-124)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(3-46)


Sequences

Toxin        


Download         Length: 141 a.a.        Molecular weight: 15468.37 Da        Isoelectric Point: 4.5876

>T193919 WP_006348208.1 NZ_CP070379:c4623381-4622959 [Streptomyces tsukubensis]
VSAAQFLIDTSALARFLRGDAEQYGWDQAAAAGLIATCPITELEFFYSARSAEDRARGVEDLRMIFGWVPVDDRAYDRAW
QVQELLTRRGQHRSAGPVDLVVAATAELQGLTLLHRDRDFDCIAAVTGQALQWFGPDPGK

Download         Length: 423 bp

>T193919 NZ_CP093283:185984-186091 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7893.87 Da        Isoelectric Point: 5.0634

>AT193919 WP_040915655.1 NZ_CP070379:c4623593-4623378 [Streptomyces tsukubensis]
MSRTVIDLDDELLADVAQALGTSTKKETVNTALREVLENRRRALALARLRAAAADGGFDLDVFDDKQSYRR

Download         Length: 216 bp

>AT193919 NZ_CP093283:c185936-185870 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB I2N1L9

Antitoxin

Source ID Structure
AlphaFold DB A0A7G3UFR9

References