Detailed information of TA system TA193916

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 171924..172539
• Replicon: plasmid p2
• Accession: NZ_CP070372
• Organism: Paracoccus sp. H4-D09

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JWJ88_RS21335
• Protein ID: WP_205296976.1
• Coordinates: 172141..172539 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JWJ88_RS21330
• Protein ID: WP_205296975.1
• Coordinates: 171924..172151 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JWJ88_RS21310	167332..168300	+	969	WP_205296972.1	extracellular solute-binding protein	-
JWJ88_RS21315	168307..170034	+	1728	WP_205296973.1	ABC transporter permease subunit	-
JWJ88_RS21320	170022..171112	+	1091	Protein_168	MBL fold metallo-hydrolase	-
JWJ88_RS21325	171072..171656	+	585	WP_205296974.1	protein phosphatase	-
JWJ88_RS21330	171924..172151	+	228	WP_205296975.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JWJ88_RS21335	172141..172539	+	399	WP_205296976.1	PIN domain-containing protein	Toxin
JWJ88_RS21340	172890..173222	+	333	WP_205296977.1	hypothetical protein	-
JWJ88_RS21345	173317..173628	-	312	WP_205296978.1	hypothetical protein	-
JWJ88_RS21350	173730..175499	-	1770	WP_205296979.1	alpha/beta fold hydrolase	-
JWJ88_RS21355	175875..176198	+	324	Protein_175	IS110 family transposase	-
JWJ88_RS21360	176524..177312	-	789	Protein_176	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..244195	244195
	-	flank	IS/Tn	-	-	176524..177150	626

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-117)

Antitoxin

• SpoVT-AbrB domain (5-37)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14655.73 Da
• Isoelectric Point: 4.3660

>T193916 WP_205296976.1 NZ_CP070372:172141-172539 [Paracoccus sp. H4-D09]
MRADFFDTNIVLYLLDDSPKADMAAGLLANGGVISVQVLNEALVNCIRKARMSWQEAGDFLAGVRELCEVVDLSARMHDV
GLAVGERYGFSVYDAMIVSAALTSDCERLYTEDLQHDQLIEGRLRVVNPFRQ

Nucleotide

• Length: 399 bp

>T193916 NZ_CP093280:c5949898-5949323 [Pseudomonas sp. T1-3-2]
ATGAGGCATTCCCCCTTCACGGCCGTCTATGACGCATGCGTTCTCTATCCCGCGCCGCTGCGGGACTTCTTGATGTGGCT
GGGGCTGTCCGGTCGGTTCCGAGCGCGCTGGAGCCAAACCATTCACGAGGAGTGGAAGCGCAACCTGCTGATCAATCGGC
CCGATCTCACCAGGGCGCAAGTCGACCGAACGTCTGACCTGATGGATCGTGCCATCCCCGATGGATTGGTAGAAGGGTAC
GAATCACTCGCGGCCGGTCTGACCTTGCCAGATCCGGACGACCGCCACGTGCTGGCCGCCGCCATTCGCTGTGGGGCGAG
TGTGATCGTGACATTCAACCAGCGAGATTTCCCGAATGAGCTGCTGGCGCCCTACGGCGTCGAATCGCAGCATCCGGATG
AGTTCGTGGACAACTTGCTGGATCTGGATGCGGCTGCAGTCGTCTCGGCCGCACAGCGCCAGCGTGCCCAACTGAGGAAC
CCGACGATCGACGTCGATCGCTATCTCGATATCTTGCTGCGCCAAGGCCTGGTCCAGACCACCAAGGTGCTTGCCAGCTA
CCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8428.61 Da
• Isoelectric Point: 8.7376

>AT193916 WP_205296975.1 NZ_CP070372:171924-172151 [Paracoccus sp. H4-D09]
MQIAKWGNSLAVRLPAELVRELGLEAGDEIELYPAEKGFAVHRRPSAEDVLADLRRFRGRLPAAERMSRNDANAR

Nucleotide

• Length: 228 bp

>AT193916 NZ_CP093280:c5950362-5949895 [Pseudomonas sp. T1-3-2]
ATGACCGTTACTGCCCAATCCAAGATGACTCTGCCCGCCGAGGGTGAGGTGAAGGCAGCCGTGCAGGGGCAACGCGCCTT
GGCTGCCTACCTCGCGACGCAGTTTGAAACTCAGCACATCCAGATTTTTGATGACCAGAAACAGGCGCACCAAGTCGAGC
TGCCGACCTCGGCTTTGCGTTTGTTAGTGGACATCTTGGCCGAACTGGCCGACGGCAATGCTGTGAAGGTGGTGCCTGTC
TATGCGGAGCTGACAACACAGGAGGCCGCGGACCTGCTCAACGTGTCCCGTCCGCATTTCGTCAAGCTGCTCGAAGATGG
GGTGTTGGCGTTTCATCGGACTGGCAAGCACCGCCGGGTGAGATTTGCCGATCTGATGCAGTACAAGGAGGCGCGTGAGC
GCGCGAGCGAGCAGGCGATGGCTGAACTCACCCAGCAGTCGCAAGAATTGGGAATGGGGTACGAATGA