Detailed information of TA system TA193900

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2313333..2313969
• Replicon: chromosome
• Accession: NZ_CP070345
• Organism: Bacillus sp. PDNC022

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0P7G713
• Locus tag: JVX94_RS12035
• Protein ID: WP_024425388.1
• Coordinates: 2313333..2313683 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: W8QJ31
• Locus tag: JVX94_RS12040
• Protein ID: WP_003214273.1
• Coordinates: 2313688..2313969 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JVX94_RS11995	2308363..2308962	-	600	WP_205183263.1	SpoIIE family protein phosphatase	-
JVX94_RS12000	2308962..2309750	-	789	WP_024427378.1	RNA polymerase sigma factor SigB	-
JVX94_RS12005	2309716..2310204	-	489	WP_048240335.1	anti-sigma B factor RsbW	-
JVX94_RS12010	2310201..2310530	-	330	WP_017358393.1	anti-sigma factor antagonist	-
JVX94_RS12015	2310590..2311597	-	1008	WP_095407804.1	PP2C family protein-serine/threonine phosphatase	-
JVX94_RS12020	2311608..2312009	-	402	WP_024427376.1	anti-sigma regulatory factor	-
JVX94_RS12025	2312012..2312380	-	369	WP_003214235.1	RsbT antagonist protein RsbS	-
JVX94_RS12030	2312385..2313215	-	831	WP_024427375.1	RsbT co-antagonist protein RsbRA	-
JVX94_RS12035	2313333..2313683	-	351	WP_024425388.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
JVX94_RS12040	2313688..2313969	-	282	WP_003214273.1	hypothetical protein	Antitoxin
JVX94_RS12045	2314263..2315429	-	1167	WP_095407803.1	alanine racemase	-
JVX94_RS12050	2315569..2316585	-	1017	WP_095407802.1	outer membrane lipoprotein carrier protein LolA	-
JVX94_RS12055	2316746..2317111	-	366	WP_205183266.1	holo-ACP synthase	-
JVX94_RS12060	2317206..2317811	+	606	WP_205183270.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8891

>T193900 WP_024425388.1 NZ_CP070345:c2313683-2313333 [Bacillus sp. PDNC022]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Nucleotide

• Length: 351 bp

>T193900 NZ_CP093271:c4711974-4711717 [Klebsiella oxytoca]
ATGAATCTATCCCGTCAGGAACAACGTACCTTACACGTTCTCGCTAAAGGTGGCCGGATCGCGCATATTCGCGATGCATC
AGGCCGCGTAACCTCTGTTGAATGCTACAGCCGCGAAGGGCTCCTGCTCAGCGACTGCACGCTCGCCGTTTTCAAAAAAC
TCAAAACCAAAAAACTCATTAAGTCCGTCAACGGGCAGCCCTACCGTATCAATACCACCGGGCTGAACAACGTTCGCGCT
CAGCTGGACAACCGCTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10864.25 Da
• Isoelectric Point: 5.2726

>AT193900 WP_003214273.1 NZ_CP070345:c2313969-2313688 [Bacillus sp. PDNC022]
MSESSATNEMKIRLPEALMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Nucleotide

• Length: 282 bp

>AT193900 NZ_CP093271:c4711706-4711155 [Klebsiella oxytoca]
ATGACAAACCATAACTTCACCTTTCACATCACCTCAGAGCGTGATGCTGATGATATTCGCGAAGTTGAAACCCGGGCCTT
CGGCTTTAGCAAAGAGGCCGACCTGGTTGCCGATTTGCTCAACGATGAAAGTGCTCACCCGAGATTATCTCTGCTGGCCA
AACATAATGGAAAAGCAGTTGGGCATATTTTGTTCACCCTGGCGACGTTCAAAGGGGAGTCTGACTCTCCGATGATGCAT
ATCCTTGCCCCCCTGGCGGTGGTGCCGGAATACCAGGGCATGGGTGTTGGCGGATTACTTATCCAAAGCGGTATTGAGCA
TCTGAAGGCAATGGGGAGCCTGGCGGTATTCGTTCTGGGACATGCCACTTATTACCCGCGGCATGGCTTTGAACCCTGTG
CCGGAGATAAAGGCTATCCGGCGCCTTATCCCATTCCCGAGGAGCATAAAGCCTGCTGGATGCTGCAGCGGCTTTCGTCC
CGGCCGCTGGGGATAACCGGTCAGATACAGTGCGCCCGGGCGTTAATGAAACCCGAGCACTGGCGCGAGTAA

Structures

Toxin

• AlphaFold DB: A0A0P7G713

Antitoxin

• AlphaFold DB: A0A081L854