Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 3660706..3661348 Replicon chromosome
Accession NZ_CP070339
Organism Bacillus cereus strain VKM B-370

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag JTF64_RS18285 Protein ID WP_000635965.1
Coordinates 3660706..3661056 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag JTF64_RS18290 Protein ID WP_000004570.1
Coordinates 3661061..3661348 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JTF64_RS18270 3657642..3658100 - 459 WP_000343552.1 SprT family protein -
JTF64_RS18275 3658296..3658412 + 117 WP_001143640.1 cortex morphogenetic protein CmpA -
JTF64_RS18280 3658470..3660638 - 2169 WP_141403412.1 RNA-binding transcriptional accessory protein -
JTF64_RS18285 3660706..3661056 - 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
JTF64_RS18290 3661061..3661348 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
JTF64_RS18295 3661659..3662828 - 1170 WP_000390595.1 alanine racemase -
JTF64_RS18300 3662946..3663896 - 951 WP_002084009.1 outer membrane lipoprotein carrier protein LolA -
JTF64_RS18305 3664053..3664412 - 360 WP_000583423.1 holo-ACP synthase -
JTF64_RS18310 3664506..3665078 + 573 WP_000908527.1 rhomboid family intramembrane serine protease -
JTF64_RS18315 3665071..3666033 - 963 WP_000961146.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T193898 WP_000635965.1 NZ_CP070339:c3661056-3660706 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T193898 NZ_CP093271:2202091-2202194 [Klebsiella oxytoca]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTTTTTTT

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT193898 WP_000004570.1 NZ_CP070339:c3661348-3661061 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT193898 NZ_CP093271:c2202201-2202056 [Klebsiella oxytoca]
AAAAGATAAAAAAAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCCTTATAAGAATAGTTTACCGTGTCAGGTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References