Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) /SpoIISA(toxin)
Location 1553738..1554716 Replicon chromosome
Accession NZ_CP070339
Organism Bacillus cereus strain VKM B-370

Toxin (Protein)


Gene name spoIISA Uniprot ID B7IX38
Locus tag JTF64_RS07625 Protein ID WP_000624981.1
Coordinates 1553979..1554716 (-) Length 246 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID B7IX39
Locus tag JTF64_RS07620 Protein ID WP_000237817.1
Coordinates 1553738..1553866 (-) Length 43 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JTF64_RS07590 1549255..1549506 + 252 WP_000821942.1 response regulator transcription factor -
JTF64_RS07595 1549647..1550252 - 606 WP_000527414.1 hypothetical protein -
JTF64_RS07600 1550278..1551087 - 810 WP_098832561.1 C39 family peptidase -
JTF64_RS07605 1551400..1552869 - 1470 WP_000287541.1 beta-Ala-His dipeptidase -
JTF64_RS07610 1553081..1553470 + 390 WP_000712655.1 YxeA family protein -
JTF64_RS07615 1553489..1553665 - 177 WP_098832560.1 stage II sporulation protein SB -
JTF64_RS07620 1553738..1553866 - 129 WP_000237817.1 hypothetical protein Antitoxin
JTF64_RS07625 1553979..1554716 - 738 WP_000624981.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
JTF64_RS07630 1554955..1555692 - 738 WP_000594159.1 2,3-diphosphoglycerate-dependent phosphoglycerate mutase -
JTF64_RS07635 1555858..1556340 - 483 WP_000191905.1 MarR family winged helix-turn-helix transcriptional regulator -
JTF64_RS07640 1556448..1556616 + 169 Protein_1527 hypothetical protein -
JTF64_RS07645 1556592..1557437 + 846 Protein_1528 alpha-keto acid decarboxylase family protein -
JTF64_RS07650 1557591..1558373 - 783 WP_000381782.1 class I SAM-dependent methyltransferase -
JTF64_RS07655 1558718..1559125 - 408 WP_000072495.1 VOC family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(10-243)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 246 a.a.        Molecular weight: 28335.09 Da        Isoelectric Point: 8.2998

>T193897 WP_000624981.1 NZ_CP070339:c1554716-1553979 [Bacillus cereus]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNVFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Download         Length: 738 bp

>T193897 NZ_CP093271:899260-899670 [Klebsiella oxytoca]
GTGGTCCTGTGGCAATCTGATTTACGCATCTCGTGGCGAGCGCAATGGTTCTCACTGTTGATGCACGGCGTGGTGGCGGC
GCTGGTGCTGGTGTTGCCCTGGCCGCTGAGCTATACCCCGCTGTGGCTGATACTGCTGTCGCTGGTGGTGTTCGACTGCG
TGCGCAGCCAACGGCGGATCCATGCCCGACAGGGGGAAATAAAGTTGCTGATTGACTCTCGCCTGCGCTGGCAAAAAGCG
GAGTGGGATATCGTCGGCACCCCGTGGGTAATCAATAGCGGAATGCTGCTGCGTCTGCGAAATACGGAAAACCAACGCAC
TCAGCATCTATGGGTGGCGGCGGATAGCATGGACGCGGGCGAGTGGCGCGATCTGCGTCGGCTGGTGTTACAGAAACCGA
CGCAAGATTAA

Antitoxin


Download         Length: 43 a.a.        Molecular weight: 4981.37 Da        Isoelectric Point: 3.9150

>AT193897 WP_000237817.1 NZ_CP070339:c1553866-1553738 [Bacillus cereus]
VVKDYDNDFFKEDNEDTTDFSLIKGDALQQVEELEEELKRKS

Download         Length: 129 bp

>AT193897 NZ_CP093271:899013-899279 [Klebsiella oxytoca]
ATGGATATTAACAATAAAGCTCGCATCCACTGGGCATGCCGCCGGGGAATGCGTGAACTCGATATCTCCATCATGCCGTT
TTTTGAGCATGAGTACGATACGCTCAGTGATGACGATAAGCGGTTGTTCATCCGCCTGCTGGAAAATGACGATCCGGACC
TGTTCAACTGGTTGATGAACCATGGCGAACCGGCTGATGCAGAACTGCAGCGGATGGTAAAATTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A6D1SWF1


Antitoxin

Source ID Structure
AlphaFold DB B7IX39

References