Detailed information of TA system TA193882

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3176450..3177018
• Replicon: chromosome
• Accession: NZ_CP070338
• Organism: Mycobacterium tuberculosis strain 11502

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: JR349_RS14745
• Protein ID: WP_003405865.1
• Coordinates: 3176450..3176824 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: JR349_RS14750
• Protein ID: WP_003405863.1
• Coordinates: 3176821..3177018 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JR349_RS14710	3172863..3173570	+	708	Protein_2910	adenylate/guanylate cyclase domain-containing protein	-
JR349_RS14715	3173522..3173731	-	210	WP_003911400.1	hypothetical protein	-
JR349_RS14720	3173753..3174463	+	711	WP_003911399.1	hypothetical protein	-
JR349_RS14725	3174478..3174801	-	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
JR349_RS14730	3175044..3175319	+	276	WP_003405867.1	hypothetical protein	-
JR349_RS14735	3175246..3175431	-	186	WP_003901093.1	hypothetical protein	-
JR349_RS14740	3175549..3176247	-	699	WP_003898733.1	hypothetical protein	-
JR349_RS14745	3176450..3176824	-	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
JR349_RS14750	3176821..3177018	-	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JR349_RS14755	3177106..3178179	-	1074	WP_003898732.1	redox-regulated ATPase YchF	-
JR349_RS14760	3178242..3179225	+	984	WP_003898731.1	hypothetical protein	-
JR349_RS14765	3179242..3180249	-	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
JR349_RS14770	3180339..3180977	+	639	WP_003898730.1	lipid droplet-associated protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T193882 WP_003405865.1 NZ_CP070338:c3176824-3176450 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T193882 NZ_CP093255:2753662-2753913 [Moellerella wisconsensis]
ATGAAAGCGGTAAGCTACAGTGAAGCCAGACAGAATTTATCAACAACAATGATTCAAACAGTTGAAGATAGAGTACCAGT
ACTGATCACGCGCCAGAATGGCGAATCATGTGTACTGATGTCTCTCGATGAATATAACTCTCTCGAAGAGACGGCATACC
TAATGAGATCACCAAAAAATGCACGCAGACTTATGGACTCGATAGAAAGCCTAAAAGATGGAAAAGGGATGGAAAGGGAA
ATTATTAAGTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT193882 WP_003405863.1 NZ_CP070338:c3177018-3176821 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT193882 NZ_CP093255:2753910-2754164 [Moellerella wisconsensis]
GTGAAGCTAACATGGTCTGCCGAAGCATGGGAAGATTACTTGTACTGGCAAGAAACGGACAGAAAAACCGTAAAAAAGAT
AAATGAGCTCATTAAGAATGCCAGTAGAACACCATTTGAAGGAAAAGGTAAACCTGAACCTCTGAAACACAATCTTGCTG
GGTTCTGGTCACGAAGAATTACGGCGGAACATCGATTAGTCTATGCCGTTAGTAATGATTCCTTGCTAATAGCATCATGT
CGTTATCACTACTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07