Detailed information of TA system TA193876

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2847956..2848643
• Replicon: chromosome
• Accession: NZ_CP070338
• Organism: Mycobacterium tuberculosis strain 11502

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: JR349_RS13280
• Protein ID: WP_003414064.1
• Coordinates: 2848248..2848643 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: JR349_RS13275
• Protein ID: WP_003414061.1
• Coordinates: 2847956..2848222 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JR349_RS13245	2843595..2844497	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
JR349_RS13250	2844566..2845318	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
JR349_RS13255	2845562..2845837	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
JR349_RS13260	2845834..2847456	-	1623	WP_003414057.1	type I restriction-modification system subunit M	-
JR349_RS13265	2847341..2847589	+	249	WP_172643166.1	hypothetical protein	-
JR349_RS13270	2847543..2847959	-	417	Protein_2624	type II toxin-antitoxin system toxin ribonuclease C21	-
JR349_RS13275	2847956..2848222	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JR349_RS13280	2848248..2848643	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
JR349_RS13285	2848640..2848909	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
JR349_RS13290	2848919..2850013	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
JR349_RS13295	2850010..2850429	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
JR349_RS13300	2850503..2850982	-	480	WP_003414073.1	dihydrofolate reductase	-
JR349_RS13305	2851053..2851853	-	801	WP_003911953.1	thymidylate synthase	-
JR349_RS13310	2852009..2852746	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T193876 WP_003414064.1 NZ_CP070338:c2848643-2848248 [Mycobacterium tuberculosis]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T193876 NZ_CP093249:2933863-2934087 [Moellerella wisconsensis]
ATGAATGAAAGACAGGAAAATATGACTAAAACAGACTATCTGATGCGTTTAAGAAAATGCACGACTATTGAAACATTAGA
GCGTGTTATTGAAAAAAATAAATATGAGCTATCTGATGATGAACTCGAACTGTTTTTTTCCGCTGCTGACCATCGTCTAG
CAGAACTAACAATGAATAAACTGTATGACAAAATTCCAGCATCTGTCTGGAAATATGTTCGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT193876 WP_003414061.1 NZ_CP070338:c2848222-2847956 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT193876 NZ_CP093249:2933448-2933816 [Moellerella wisconsensis]
ATGGATGAATATTCACCCAGATATTACGATACTGCTGAATTAAAGTATTTATGTGATAGCCTTAACAATGATGCGATATT
ATCGCTACATAAGACTAAAACGCATTGGATTAATGATTTAAGTTCACAGCAAAGTACGAATTTGAATGAGTTAATAGAGC
ATGTGGCTGCATTTTTTTGGAAATTTAAGATAAAATACCCTAGTGAACGCTTAGTCATCTCTCTAGTTGATGAATATCTT
GATGAAACTTATAATTTATTTGGTAGTCCATTAATCACATTAAATGAATTTTCAGATTGGCAAAAAATGAATACTCACTT
AATTGATGAATTAAATAACGATTTAAAGTGTTCAGTAGCCAAACTATGA

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64