Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2645730..2646361 Replicon chromosome
Accession NZ_CP070338
Organism Mycobacterium tuberculosis strain 11502

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag JR349_RS12165 Protein ID WP_003413174.1
Coordinates 2645984..2646361 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag JR349_RS12160 Protein ID WP_003413167.1
Coordinates 2645730..2645987 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JR349_RS12130 2641645..2641959 + 315 WP_009937839.1 hypothetical protein -
JR349_RS12135 2642255..2643466 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
JR349_RS12140 2643593..2644252 + 660 WP_003902296.1 hypothetical protein -
JR349_RS12145 2644249..2644911 + 663 WP_003905878.1 hypothetical protein -
JR349_RS12150 2644908..2645185 + 278 Protein_2404 type II toxin-antitoxin system VapB family antitoxin -
JR349_RS12155 2645278..2645691 + 414 WP_003413164.1 PIN domain nuclease -
JR349_RS12160 2645730..2645987 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein Antitoxin
JR349_RS12165 2645984..2646361 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
JR349_RS12170 2646377..2646751 - 375 WP_003413177.1 hypothetical protein -
JR349_RS12175 2646851..2647246 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
JR349_RS12180 2647243..2647488 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
JR349_RS12185 2647899..2648318 - 420 WP_003413190.1 A24 family peptidase -
JR349_RS12190 2648330..2649139 - 810 WP_003413193.1 shikimate dehydrogenase -
JR349_RS12195 2649136..2650389 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
JR349_RS12200 2650382..2650894 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T193872 WP_003413174.1 NZ_CP070338:2645984-2646361 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T193872 NZ_CP093246:125274-125663 [Moellerella wisconsensis]
GTGGCGTTCTATAAAATCTCATCTTTTGTCCGAAGCACCAAAAAAATACCTATAACAGACAAACAGTTACTTGATGCTGC
TCATGAAGTTCAACAAGGACAATTTGAAGCTGATTTAGGCGGTGGAGTGATAAAAAAACGCCTTGCAATGAAAGGGCAAG
GCAAAAGTGGAAGTGTTAGGGTTATTATCTTTTTTAAGATAAACAATCATCTTTTTTTTGCAGATGGATGGACCAAAAAC
ACGGTCAATGCCAAAGGTACAAAAGAGATTGAAGATGATGAGTTGGAAACCTATAAAAAATTAGCCGCTGAGTTTCTGAA
CTTTGATGGAAAAATCATTAACAATTTAATTTCTAAAGGAATTCTGGAGGAAATTACTGATGAACAATAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT193872 WP_003413167.1 NZ_CP070338:2645730-2645987 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT193872 NZ_CP093246:125653-125982 [Moellerella wisconsensis]
ATGAACAATAAACTTAATAACATGCTTGAATTGGCTAAAGAGTTAAATGCAGCTGGTGCATCACCTGAAAGCCTGGTTAA
AGATATTGAAAGCACCATCCGTGCTCGTGAAATTAACGATAAAATAAAAGAAGTTTCAGCGATGAGCGGCTCTGAGATCA
AGATGTTGCGGACTAAATATTGTATGTCTCAATCTGTGCTCGCTCGTGCATTGGGAATGTCGAAAGAGAGCATATCCAAA
TGGGAGCGAGGAGAAAAGAAACCAAGCGGCCCTGCATTGAGGATGCTACGTATTCTTGAAAGCAAAGGCCCAGAAGTTTT
AGTGGTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References