Detailed information of TA system TA193864

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2029242..2029868
• Replicon: chromosome
• Accession: NZ_CP070338
• Organism: Mycobacterium tuberculosis strain 11502

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: JR349_RS09290
• Protein ID: WP_003410075.1
• Coordinates: 2029470..2029868 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A806JLL8
• Locus tag: JR349_RS09285
• Protein ID: WP_003911750.1
• Coordinates: 2029242..2029469 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JR349_RS09275	2027281..2027625	-	345	WP_003410065.1	ferredoxin family protein	-
JR349_RS09280	2027814..2028983	-	1170	WP_003899126.1	ATP-binding protein	-
JR349_RS09285	2029242..2029469	+	228	WP_003911750.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JR349_RS09290	2029470..2029868	+	399	WP_003410075.1	PIN domain nuclease	Toxin
JR349_RS09295	2030051..2030482	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
JR349_RS09300	2030583..2031017	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
JR349_RS09305	2031454..2031633	-	180	Protein_1845	hypothetical protein	-
JR349_RS09310	2031694..2032341	+	648	WP_071854215.1	transposase	-
JR349_RS09315	2032295..2032885	+	591	WP_003899131.1	IS110 family transposase	-
JR349_RS09320	2033013..2034269	-	1257	WP_003902247.1	HNH endonuclease	-
JR349_RS09325	2034623..2034823	+	201	Protein_1849	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T193864 WP_003410075.1 NZ_CP070338:2029470-2029868 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T193864 NZ_CP093244:c3991-3716 [Moellerella wisconsensis]
ATGCTTATCCCTGTACAGTCCAGTGCCTTTAAGTCGGATGTGAAGAGACAAAAGAAACGCGGTAAGGATATGGGGAAGTT
AAAAACACTCATTGAATTACTGGTTAATGAGAACGATATACCACCGGAGTATGTTGATCATCCTTTATCAGGAAACTGGC
GGGGGTACCGTGATGCTCATATTGAAGGTGACTGGTTACTGATTTATAAGGTTGATGATGATGCGTTAAGGCTCGCCAGA
ACCGGTACGCATCAGGACATTTTCAGTAATTATTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8346.16 Da
• Isoelectric Point: 4.1621

>AT193864 WP_003911750.1 NZ_CP070338:2029242-2029469 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT193864 NZ_CP093244:c4248-3991 [Moellerella wisconsensis]
ATGATCGCTGACGCAACTGTTAAAGCTAGAATCCCGCAGGATGTTAAAGACCGAGCGGTACAGGCACTTGAAAAGATAGG
ACTGAATACTTCGGATGTGATCCGTATGGTTATGATGCGTGTTGCTGACGAGGGTAAGCTTCCTTTTGAGGTAAAAACAC
CGAATAAAAAAACGCAAGTCGCCATGTCTGAATTAGATTCAGGAAAAGGTCAGCGTTTTGATTCTGTTGATGCTCTTTTT
GATGATTTGGGTATCTGA

Structures

Toxin

• PDB: 4CHG

Antitoxin

• AlphaFold DB: A0A806JLL8