Detailed information of TA system TA193858

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1963103..1963806
• Replicon: chromosome
• Accession: NZ_CP070338
• Organism: Mycobacterium tuberculosis strain 11502

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: JR349_RS08890
• Protein ID: WP_003409778.1
• Coordinates: 1963103..1963432 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: JR349_RS08895
• Protein ID: WP_003409780.1
• Coordinates: 1963429..1963806 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JR349_RS08870	1959486..1960556	+	1071	WP_003902252.1	epoxide hydrolase EphB	-
JR349_RS08875	1960553..1961068	+	516	WP_003409718.1	flavin reductase family protein	-
JR349_RS08880	1961065..1962126	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
JR349_RS08885	1962123..1962893	+	771	WP_003409775.1	SDR family oxidoreductase	-
JR349_RS08890	1963103..1963432	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JR349_RS08895	1963429..1963806	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
JR349_RS08900	1963803..1964393	-	591	WP_003409784.1	SEC-C domain-containing protein	-
JR349_RS08905	1964448..1965812	+	1365	WP_003910878.1	HNH endonuclease	-
JR349_RS08910	1965967..1966419	-	453	WP_003899095.1	lipoprotein	-
JR349_RS08915	1966483..1966884	+	402	WP_003409869.1	hypothetical protein	-
JR349_RS08920	1966877..1967059	-	183	WP_003409870.1	hypothetical protein	-
JR349_RS08925	1967173..1967523	-	351	WP_003409871.1	hypothetical protein	-
JR349_RS08930	1967534..1968436	-	903	WP_003899097.1	hypothetical protein	-
JR349_RS08935	1968457..1968648	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T193858 WP_003409778.1 NZ_CP070338:c1963432-1963103 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T193858 NZ_CP093242:c3177804-3177538 [Moellerella wisconsensis]
TTGGTCTGGACGATTAATTATTCTGAACGGGCGTTAAAATCTCTACGAAAAATGGATAAACAGAGTGCTCGCCGTATTGT
CGATTTTATGGGTTTACGCATTGCAGCTTTAGATGATCCCCGACAGTTAGGCAAATCACTCAAAGGTGAACTAGGTGAGT
TCTGGCGTTATAGAGTTGGTGACTATCGGATACTATGCGAGATAAGGGATGATGAACTTGTCATTTTGGCTGCTACAATA
GGACATCGTCGTGAGATATATGAGTAA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT193858 WP_003409780.1 NZ_CP070338:c1963806-1963429 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT193858 NZ_CP093242:c3178012-3177788 [Moellerella wisconsensis]
ATGTTAGCTATTCGTTTATCTGATGACATTGAAACACGTCTGGAGTCACTTGCAAAACAGACAGGAAGAACCAAAACGTT
CTATGCGAGAGAAGCTATTCTCACCCACTTGGAAGACTTGGAAGACTACTATTTATCCGCTGAAACCATTGCAAAAATTC
GTAGCGGTGAAGAGTTGATTCATTCGTCTGAAGAGGTGAGAAAAACACTTGGTCTGGACGATTAA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8