Detailed information of TA system TA193806

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 63697..64226
• Replicon: chromosome
• Accession: NZ_CP070305
• Organism: Staphylococcus sp. SM3655

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: JQN76_RS00355
• Protein ID: WP_000621175.1
• Coordinates: 63697..64059 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: JQN76_RS00360
• Protein ID: WP_000948331.1
• Coordinates: 64056..64226 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQN76_RS00335	60675..61445	-	771	WP_205397779.1	RNA polymerase sigma factor SigB	-
JQN76_RS00340	61420..61899	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
JQN76_RS00345	61901..62227	-	327	WP_001052491.1	anti-sigma factor antagonist	-
JQN76_RS00350	62346..63347	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
JQN76_RS00355	63697..64059	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JQN76_RS00360	64056..64226	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
JQN76_RS00365	64311..65459	-	1149	WP_001281154.1	alanine racemase	-
JQN76_RS00370	65525..65884	-	360	WP_000581197.1	holo-ACP synthase	-
JQN76_RS00375	65888..66379	-	492	WP_001205912.1	PH domain-containing protein	-
JQN76_RS00380	66366..67949	-	1584	Protein_70	PH domain-containing protein	-
JQN76_RS00385	67942..68421	-	480	WP_001836941.1	hypothetical protein	-
JQN76_RS00390	68630..69190	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T193806 WP_000621175.1 NZ_CP070305:c64059-63697 [Staphylococcus sp. SM3655]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T193806 NZ_CP093222:1931440-1931628 [Staphylococcus epidermidis]
ATGAGCTTACATTTCCAAATTTTGCTGTGGCTTTCTATCTTATTTATCATCGCAGGAACGATATTATTGGTAACAATGCT
TAAAACTAAAAAAGAAGAACGAAAAGAATCTTATCTAGGCTTTACTGTAATTTTCTTAATCTTTGGTTTTGCCATCTTAA
TTTATACTTTTATATTCGGAATTTTATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT193806 WP_000948331.1 NZ_CP070305:c64226-64056 [Staphylococcus sp. SM3655]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT193806 NZ_CP093222:1931158-1931415 [Staphylococcus epidermidis]
ATGAAATTAAACAAAGCAAATATATTCAATTTGATATTCACCATTTTATTTTTTTCATTTAACATTCTCATAACCTATAA
CGCAAATATAGATTATAAGTTATGGTTAATACCTGGTTTAGCAATTTGTGGTTTTGCTCTTTTTTCAAGCTTGACCTTAG
TAATTATCTATAGTGATTTATTCAGTGAAATATTATTTTTTATCAACATTATCTTAGCACTGTATTACATTTATCCGATT
TTTTATGAATTTGTCTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1