Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 8256..8899 Replicon plasmid pESA136_4
Accession NZ_CP070300
Organism Escherichia albertii strain Sample 165

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag JRC44_RS25725 Protein ID WP_001044768.1
Coordinates 8256..8672 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag JRC44_RS25730 Protein ID WP_001261287.1
Coordinates 8669..8899 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JRC44_RS25710 (4755) 4755..5345 - 591 WP_000194575.1 hypothetical protein -
JRC44_RS25715 (5345) 5345..5602 - 258 WP_000343085.1 hypothetical protein -
JRC44_RS25720 (5956) 5956..8094 + 2139 WP_000350638.1 AAA family ATPase -
JRC44_RS25725 (8256) 8256..8672 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
JRC44_RS25730 (8669) 8669..8899 - 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
JRC44_RS25735 (9195) 9195..9485 + 291 WP_000111771.1 hypothetical protein -
JRC44_RS25740 (9475) 9475..10374 + 900 WP_000963206.1 nucleotide-binding protein -
JRC44_RS25745 (10424) 10424..12649 - 2226 WP_000698737.1 P-loop NTPase fold protein -
JRC44_RS25750 (12651) 12651..13739 - 1089 WP_000952217.1 transcriptional repressor PifC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-55 / blaTEM-1B / fosA3 - 1..97847 97847


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T193799 WP_001044768.1 NZ_CP070300:c8672-8256 [Escherichia albertii]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T193799 NZ_CP093221:3734098-3734205 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT193799 WP_001261287.1 NZ_CP070300:c8899-8669 [Escherichia albertii]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT193799 NZ_CP093221:c3734051-3733985 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References