Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 200357..201980 Replicon plasmid pESA136_1
Accession NZ_CP070297
Organism Escherichia albertii strain Sample 165

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag JRC44_RS24440 Protein ID WP_000088046.1
Coordinates 200357..201682 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag JRC44_RS24445 Protein ID WP_024136327.1
Coordinates 201687..201980 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JRC44_RS24400 (JRC44_24400) 195378..195734 + 357 WP_065311124.1 H-NS family nucleoid-associated regulatory protein -
JRC44_RS24405 (JRC44_24405) 196076..196279 - 204 WP_001194555.1 hypothetical protein -
JRC44_RS24410 (JRC44_24410) 196341..197198 - 858 WP_208629815.1 HNH endonuclease signature motif containing protein -
JRC44_RS24415 (JRC44_24415) 197214..197522 - 309 WP_001224687.1 hypothetical protein -
JRC44_RS24420 (JRC44_24420) 198071..198496 - 426 WP_001043843.1 hypothetical protein -
JRC44_RS24425 (JRC44_24425) 198750..199565 - 816 WP_208629816.1 HNH endonuclease signature motif containing protein -
JRC44_RS24430 (JRC44_24430) 199578..199988 - 411 WP_000985911.1 hypothetical protein -
JRC44_RS24435 (JRC44_24435) 200090..200296 - 207 WP_000134171.1 hypothetical protein -
JRC44_RS24440 (JRC44_24440) 200357..201682 - 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
JRC44_RS24445 (JRC44_24445) 201687..201980 - 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
JRC44_RS24450 (JRC44_24450) 202584..202976 - 393 WP_000341066.1 hypothetical protein -
JRC44_RS24455 (JRC44_24455) 203349..203690 + 342 WP_001151575.1 hypothetical protein -
JRC44_RS24460 (JRC44_24460) 203782..203973 + 192 WP_000398480.1 hypothetical protein -
JRC44_RS24465 (JRC44_24465) 204023..204304 + 282 WP_000077926.1 hypothetical protein -
JRC44_RS26880 204645..204776 - 132 WP_000374059.1 hypothetical protein -
JRC44_RS24470 (JRC44_24470) 204867..206108 - 1242 WP_001053338.1 VWA domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA14 / ARR-3 / mph(A) / qnrS1 / blaCTX-M-55 / blaTEM-1B / blaLAP-2 / aac(3)-IId / ant(3'')-Ia / lnu(F) / sul3 / tet(A) / aph(6)-Id / floR / aph(3')-Ia - 1..266043 266043


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T193792 WP_000088046.1 NZ_CP070297:c201682-200357 [Escherichia albertii]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T193792 NZ_CP093221:2911906-2912160 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT193792 WP_024136327.1 NZ_CP070297:c201980-201687 [Escherichia albertii]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT193792 NZ_CP093221:2911658-2911909 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References