Detailed information of TA system TA193766

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 51141..51742
• Replicon: plasmid pESA139_3
• Accession: NZ_CP070295
• Organism: Escherichia albertii strain Sample 166

Toxin (Protein)

• Gene name: doc
• Uniprot ID: V0AJ64
• Locus tag: JRC43_RS24940
• Protein ID: WP_001216034.1
• Coordinates: 51141..51521 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: JRC43_RS24945
• Protein ID: WP_001190712.1
• Coordinates: 51521..51742 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JRC43_RS24915 (JRC43_24915)	46581..48065	-	1485	WP_000124159.1	hypothetical protein	-
JRC43_RS24920 (JRC43_24920)	48065..49258	-	1194	WP_000219605.1	terminase	-
JRC43_RS24925 (JRC43_24925)	49345..49797	-	453	WP_001312282.1	late promoter-activating protein (Gp10)	-
JRC43_RS24930 (JRC43_24930)	49886..50929	-	1044	WP_000648827.1	DUF968 domain-containing protein	-
JRC43_RS24935 (JRC43_24935)	50957..51136	-	180	WP_078182672.1	PdcA protein	-
JRC43_RS24940 (JRC43_24940)	51141..51521	-	381	WP_001216034.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
JRC43_RS24945 (JRC43_24945)	51521..51742	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JRC43_RS24950 (JRC43_24950)	51815..52204	-	390	WP_000506730.1	S24 family peptidase	-
JRC43_RS24955 (JRC43_24955)	52379..52735	+	357	WP_208634916.1	DNA-binding protein	-
JRC43_RS24960 (JRC43_24960)	52732..52950	+	219	WP_208634917.1	hypothetical protein	-
JRC43_RS24965 (JRC43_24965)	52951..53307	+	357	WP_001062545.1	hypothetical protein	-
JRC43_RS24970 (JRC43_24970)	54383..54745	-	363	WP_208634918.1	hypothetical protein	-
JRC43_RS25770	55588..55695	-	108	Protein_58	hypothetical protein	-
JRC43_RS25775	55906..56040	-	135	Protein_59	hypothetical protein	-
JRC43_RS24980 (JRC43_24980)	56037..56402	-	366	WP_001229294.1	HNH endonuclease signature motif containing protein	-
JRC43_RS24985 (JRC43_24985)	56404..56622	-	219	WP_114211923.1	DUF4014 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..98546	98546

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13615.32 Da
• Isoelectric Point: 5.1514

>T193766 WP_001216034.1 NZ_CP070295:c51521-51141 [Escherichia albertii]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T193766 NZ_CP093212:870424-870786 [Staphylococcus epidermidis]
ATGATTAGAAGAGGAGATGTTTATTTAGCGGATTTATCACCAGTTCAAGGGTCTGAACAAGGGGGAGTAAGACCTGTAGT
TATCATTCAAAATGATACTGGTAATAAATATAGTCCAACTGTAATTGTAGCTGCGATTACTGGTAGGATTAATAAAGCGA
AAATACCAACCCACGTAGAAATTGAAAAGAAAAAGTATAAATTAGACAAAGATTCAGTTATTCTTCTTGAACAAATTAGA
ACACTAGATAAAAAGCGTTTAAAAGAAAAATTAACATTTTTATCAGAGAGTAAAATGATAGAGGTTGATAATGCCTTAGA
TATTAGTTTGGGATTAAATAACTTTGATCATCATAAATCTTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT193766 WP_001190712.1 NZ_CP070295:c51742-51521 [Escherichia albertii]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT193766 NZ_CP093212:870257-870427 [Staphylococcus epidermidis]
ATGTTATCTTTTAATCAAAATAGAAACCACAGTCTTGAACAATCTTTAAAAGAAGGTTATGCACAAATGGCCGATTTAAA
CCTCTCCCTAGCAACAGAAGCTTTTCCGATAGAGTGTGAAGCTTGTGATTGCAATGAATCACATTTAATATCTAATTCAA
AGAATGAATGA

Structures

Toxin

• AlphaFold DB: V0AJ64

Antitoxin

• AlphaFold DB: A0A829CJB6