Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 29572..30215 Replicon plasmid pESA139_1
Accession NZ_CP070293
Organism Escherichia albertii strain Sample 166

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag JRC43_RS23640 Protein ID WP_001044768.1
Coordinates 29799..30215 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag JRC43_RS23635 Protein ID WP_001261287.1
Coordinates 29572..29802 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JRC43_RS23615 (26183) 26183..26980 - 798 WP_000544830.1 IS21-like element IS21 family helper ATPase IstB -
JRC43_RS23620 (26980) 26980..28152 - 1173 WP_000952373.1 IS21-like element IS21 family transposase -
JRC43_RS23625 (28226) 28226..28996 - 771 WP_244259568.1 nucleotide-binding protein -
JRC43_RS23630 (28986) 28986..29276 - 291 WP_000111771.1 hypothetical protein -
JRC43_RS23635 (29572) 29572..29802 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
JRC43_RS23640 (29799) 29799..30215 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
JRC43_RS23645 (30377) 30377..32515 - 2139 WP_208634898.1 AAA family ATPase -
JRC43_RS23650 (32869) 32869..33126 + 258 WP_000343085.1 hypothetical protein -
JRC43_RS23655 (33126) 33126..33716 + 591 WP_000194575.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid fosA3 / blaTEM-1B / blaCTX-M-55 - 1..107432 107432


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T193756 WP_001044768.1 NZ_CP070293:29799-30215 [Escherichia albertii]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T193756 NZ_CP093208:835825-836034 [Staphylococcus epidermidis]
ATGAAATTGAAAAAAGGATTGATAAATGTTTCAGGTATTAAAACAACTGAACAAGCTAAACAGCTCAAAGATCATTTATC
TAAGATGATAGGCATCAATAGTGTGGATATCGATTACCAAATGAATGAGATTCGAGTAGAATTTGACACTCCTGCAAATT
TAAATAACATTGAAAAAGAAATTTACGATTACGGATTTCGTATTTTGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT193756 WP_001261287.1 NZ_CP070293:29572-29802 [Escherichia albertii]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT193756 NZ_CP093208:835525-835818 [Staphylococcus epidermidis]
ATGGCAGACATGGAGAGTGCACATCACTCAGAGCAAATAAAAACAAATTTAAAATCAAGATTAAATAGAATAGAAGGTCA
AGTAAGAGCAATAAATCGTATGATTGATGATGACGTTTACTGTGATGATGTACTAACTCAAATTAGAGCAACACGTTCAG
CATTGAATAGTGTAGCTACTAAATTACTAGACCATCACATGAAAGGTTGTATTATGGAAAAAATAAACGACGGTGCGCAA
GAAGAAGCAATGGATGAATTGCTCGTGACATTCCAAAAATTAATAAAAGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References