Detailed information of TA system TA193695

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5250..5875
• Replicon: plasmid pESA118_1
• Accession: NZ_CP070288
• Organism: Escherichia albertii strain Sample 168

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JRC45_RS23540
• Protein ID: WP_000911317.1
• Coordinates: 5250..5648 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L4J1D2
• Locus tag: JRC45_RS23545
• Protein ID: WP_000450532.1
• Coordinates: 5648..5875 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JRC45_RS23520 (736)	736..1119	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
JRC45_RS23530 (2104)	2104..2835	+	732	WP_001523488.1	conjugal transfer complement resistance protein TraT	-
JRC45_RS23535 (3088)	3088..5241	+	2154	WP_208625714.1	type IV conjugative transfer system coupling protein TraD	-
JRC45_RS23540 (5250)	5250..5648	-	399	WP_000911317.1	type II toxin-antitoxin system VapC family toxin	Toxin
JRC45_RS23545 (5648)	5648..5875	-	228	WP_000450532.1	toxin-antitoxin system antitoxin VapB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	espL2 / espL2	1..49739	49739

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14857.14 Da
• Isoelectric Point: 8.5264

>T193695 WP_000911317.1 NZ_CP070288:c5648-5250 [Escherichia albertii]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Nucleotide

• Length: 399 bp

>T193695 NZ_CP093154:c4234938-4234654 [Enterobacter asburiae]
ATGACTTATGAACTGGCATTTGACCCGAGGGCGTTAAAGGAGTGGAGCAAGCTGGGGCAGACTGTAAAAGACCAGTTTAA
GAAGAAACTTGCGGACGTGCTGGTGAACCCCAGGGTCGAATCGGCCCGCCTGCATGGTCTGCCGGATTGCTACAAAATAA
AACTCAGAACGCAGGGTTATCGTTTGGTCTATCAGGTTCAGGACAACGTTGTAACGGTATTTGTTATCGCTATCGGTAAG
CGAGAGAAATCAGCCGTTTATCATGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8512.55 Da
• Isoelectric Point: 4.5358

>AT193695 WP_000450532.1 NZ_CP070288:c5875-5648 [Escherichia albertii]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT193695 NZ_CP093154:c4235170-4234928 [Enterobacter asburiae]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAACGAGGCTTATGCCGTGCTGGAAAAACTCAATATCACCCC
AACGGAAGCCGTGCGATTACTGTTTCAGTACGTCGCGGAGACGGGACGTATGCCGGTGAAAACGGTCACGATTAGCGACA
GTGAAGATGCGCTGATACAGACGGTCCGGGAGCGACTGGCGAATCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A829CHF5