Detailed information of TA system TA193693

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4565706..4566322
• Replicon: chromosome
• Accession: NZ_CP070287
• Organism: Escherichia albertii strain Sample 168

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JRC45_RS22545
• Protein ID: WP_204628897.1
• Coordinates: 4565706..4566080 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B1EQU6
• Locus tag: JRC45_RS22550
• Protein ID: WP_001275524.1
• Coordinates: 4566080..4566322 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JRC45_RS22530 (4563209)	4563209..4564111	+	903	WP_000331386.1	formate dehydrogenase O subunit beta	-
JRC45_RS22535 (4564108)	4564108..4564743	+	636	WP_000829019.1	formate dehydrogenase cytochrome b556 subunit	-
JRC45_RS22540 (4564740)	4564740..4565669	+	930	WP_000027704.1	formate dehydrogenase accessory protein FdhE	-
JRC45_RS22545 (4565706)	4565706..4566080	-	375	WP_204628897.1	type II toxin-antitoxin system VapC family toxin	Toxin
JRC45_RS22550 (4566080)	4566080..4566322	-	243	WP_001275524.1	CopG family transcriptional regulator	Antitoxin
JRC45_RS22555 (4566550)	4566550..4566768	-	219	WP_001314326.1	CopG family transcriptional regulator	-
JRC45_RS22565 (4567944)	4567944..4568222	-	279	WP_032278934.1	hypothetical protein	-
JRC45_RS22570 (4568284)	4568284..4568496	-	213	WP_000197774.1	hypothetical protein	-
JRC45_RS22575 (4568581)	4568581..4568871	-	291	WP_000356397.1	NadS family protein	-
JRC45_RS22580 (4568872)	4568872..4569183	-	312	WP_000897302.1	hypothetical protein	-
JRC45_RS22585 (4569412)	4569412..4570320	+	909	WP_059239594.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4567010..4567513	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (15-48)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13946.17 Da
• Isoelectric Point: 9.4803

>T193693 WP_204628897.1 NZ_CP070287:c4566080-4565706 [Escherichia albertii]
MAKRTALFDTNILIDLFSGRIEAKQALEAWPLQNAISLVTWMEVLVGAKKYNQEHRTRIAMSAFNVIGISQDIAERSVAL
RQEYRMKLPDAIILATAQIYGFELVTRNTRDFAGIAGVITPYQS

Nucleotide

• Length: 375 bp

>T193693 NZ_CP093154:3661235-3661453 [Enterobacter asburiae]
ATGTCTGATAAACCACTCACAAAAATCGATTATTTGATGCGCTTACGACGTTGTCAGTCAATTGACACGCTCGAGCGCGT
CATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCGGTATTTTATTCAGCCGCTGACCATCGTCTTGCTGAAC
TGACAATGAATAAGCTGTATGACAAAATCCCTACTTCTGTGTGGAAATTTGTCCGTTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9256.53 Da
• Isoelectric Point: 4.3774

>AT193693 WP_001275524.1 NZ_CP070287:c4566322-4566080 [Escherichia albertii]
MSAMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVDQYLENQSKTTISSALGIWQGCEEDGVEYQRKLREEW

Nucleotide

• Length: 243 bp

>AT193693 NZ_CP093154:3660833-3661207 [Enterobacter asburiae]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGCAACCATGGCTGGGTAAACGACCCAACGTCTGCCATCAATTTACAGTTGAATGAGCTGATAGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGTTACGGCATTAACGCTCAGGATTTGCAAAAATGGCGTAAATCGGGAAATCGGCT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCGGTGAGTCTTTCCTGTTAA

Structures

Antitoxin

• AlphaFold DB: B1EQU6