Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 114581..115106 Replicon plasmid pESA134_2
Accession NZ_CP070286
Organism Escherichia albertii strain Sample 169

Toxin (Protein)


Gene name ccdB Uniprot ID -
Locus tag JRC46_RS24645 Protein ID WP_204678074.1
Coordinates 114801..115106 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID -
Locus tag JRC46_RS24640 Protein ID WP_204678073.1
Coordinates 114581..114799 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JRC46_RS24615 (109754) 109754..110875 - 1122 WP_208629374.1 hypothetical protein -
JRC46_RS24620 (111349) 111349..112938 - 1590 WP_204619574.1 IS66 family transposase -
JRC46_RS24625 (112958) 112958..113305 - 348 WP_204619037.1 IS66 family insertion sequence element accessory protein TnpB -
JRC46_RS24630 (113302) 113302..113721 - 420 WP_204619039.1 transposase -
JRC46_RS24635 (113807) 113807..114031 - 225 Protein_124 type II toxin-antitoxin system VapB family antitoxin -
JRC46_RS24640 (114581) 114581..114799 + 219 WP_204678073.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
JRC46_RS24645 (114801) 114801..115106 + 306 WP_204678074.1 type II toxin-antitoxin system toxin CcdB Toxin
JRC46_RS24650 (115107) 115107..115904 + 798 Protein_127 site-specific integrase -
JRC46_RS24655 (116161) 116161..116361 + 201 Protein_128 site-specific integrase -
JRC46_RS24660 (116407) 116407..117150 - 744 Protein_129 IS3 family transposase -
JRC46_RS24665 (117155) 117155..117334 - 180 Protein_130 IS66 family insertion sequence element accessory protein TnpB -
JRC46_RS24670 (117321) 117321..117632 - 312 WP_204620362.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - espC / espC 1..176494 176494
- inside IScluster/Tn - - 101169..117159 15990


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11684.50 Da        Isoelectric Point: 5.8430

>T193668 WP_204678074.1 NZ_CP070286:114801-115106 [Escherichia albertii]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVLVGDESWRMMTTDMSSVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T193668 NZ_CP093068:c4583741-4583568 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCACTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8399.41 Da        Isoelectric Point: 5.0506

>AT193668 WP_204678073.1 NZ_CP070286:114581-114799 [Escherichia albertii]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMVEVARFIEMNGSFADENRNW

Download         Length: 219 bp

>AT193668 NZ_CP093068:c4584065-4583769 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References