Detailed information of TA system TA193665

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 8846..9471
• Replicon: plasmid pESA134_1
• Accession: NZ_CP070285
• Organism: Escherichia albertii strain Sample 169

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JRC46_RS23390
• Protein ID: WP_204620320.1
• Coordinates: 8846..9244 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: D6IDB0
• Locus tag: JRC46_RS23395
• Protein ID: WP_000450523.1
• Coordinates: 9244..9471 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JRC46_RS25280	5359..6519	+	1161	WP_244259614.1	hypothetical protein	-
JRC46_RS23375 (JRC46_23375)	6534..7184	+	651	WP_001744120.1	hypothetical protein	-
JRC46_RS23380 (JRC46_23380)	7256..7993	+	738	WP_000926075.1	conjugal transfer complement resistance protein TraT	-
JRC46_RS23385 (JRC46_23385)	8461..8829	+	369	WP_244259628.1	DUF2726 domain-containing protein	-
JRC46_RS23390 (JRC46_23390)	8846..9244	-	399	WP_204620320.1	tRNA(fMet)-specific endonuclease VapC	Toxin
JRC46_RS23395 (JRC46_23395)	9244..9471	-	228	WP_000450523.1	toxin-antitoxin system antitoxin VapB	Antitoxin
JRC46_RS23400 (JRC46_23400)	9604..11793	+	2190	WP_204620319.1	type IV conjugative transfer system coupling protein TraD	-
JRC46_RS23405 (JRC46_23405)	11793..13904	+	2112	WP_244259627.1	MobF family relaxase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	stcE / exeE / exeG	1..120691	120691

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15034.35 Da
• Isoelectric Point: 8.2830

>T193665 WP_204620320.1 NZ_CP070285:c9244-8846 [Escherichia albertii]
MLKFMLDTNICIFTIKNKPSHIRERFNLNSGRMCISSVTVMELVYGAEKSQFPERNLAIIEGFISRLDVLDYDYAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERIAGLRLEDWC

Nucleotide

• Length: 399 bp

>T193665 NZ_CP093068:3988683-3988961 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8398.39 Da
• Isoelectric Point: 4.3719

>AT193665 WP_000450523.1 NZ_CP070285:c9471-9244 [Escherichia albertii]
METTVFLSNRSQAVRLPKAVALPEDVKKVDVIAVGRTRIITPAGESWDSWFDGDSVSADFMNDREQPAVQERESF

Nucleotide

• Length: 228 bp

>AT193665 NZ_CP093068:3988420-3988680 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A7L6LFS1