Detailed information of TA system TA193663

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4563195..4563796
• Replicon: chromosome
• Accession: NZ_CP070284
• Organism: Escherichia albertii strain Sample 169

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JRC46_RS22410
• Protein ID: WP_204619762.1
• Coordinates: 4563195..4563554 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B1EQU6
• Locus tag: JRC46_RS22415
• Protein ID: WP_001275524.1
• Coordinates: 4563554..4563796 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JRC46_RS22395 (4560698)	4560698..4561600	+	903	WP_204619761.1	formate dehydrogenase subunit beta	-
JRC46_RS22400 (4561597)	4561597..4562232	+	636	WP_000829019.1	formate dehydrogenase cytochrome b556 subunit	-
JRC46_RS22405 (4562229)	4562229..4563158	+	930	WP_000027704.1	formate dehydrogenase accessory protein FdhE	-
JRC46_RS22410 (4563195)	4563195..4563554	-	360	WP_204619762.1	type II toxin-antitoxin system VapC family toxin	Toxin
JRC46_RS22415 (4563554)	4563554..4563796	-	243	WP_001275524.1	CopG family transcriptional regulator	Antitoxin
JRC46_RS22420 (4564014)	4564014..4564232	-	219	WP_001251292.1	CopG family transcriptional regulator	-
JRC46_RS22425 (4564647)	4564647..4564925	-	279	WP_001296612.1	hypothetical protein	-
JRC46_RS22430 (4564987)	4564987..4565199	-	213	WP_000197774.1	hypothetical protein	-
JRC46_RS22435 (4565284)	4565284..4565574	-	291	WP_000356397.1	NadS family protein	-
JRC46_RS22440 (4565575)	4565575..4565886	-	312	WP_000897302.1	hypothetical protein	-
JRC46_RS22445 (4566115)	4566115..4567023	+	909	WP_204619763.1	alpha/beta hydrolase	-
JRC46_RS22450 (4567087)	4567087..4568028	-	942	WP_204620272.1	bifunctional GNAT family N-acetyltransferase/hotdog fold thioesterase	-
JRC46_RS22455 (4568073)	4568073..4568510	-	438	WP_000560977.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4541011..4565886	24875

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-110)

Antitoxin

• Ribbon-helix-helix protein, CopG (15-48)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13496.66 Da
• Isoelectric Point: 9.4803

>T193663 WP_204619762.1 NZ_CP070284:c4563554-4563195 [Escherichia albertii]
MAKRTALFDTNILIDLFSGRIEAKQALEAWPLQNAISLVTWMEVLVGAKKYNQEHRTRIAMSAFNVIGISQDIAERSVAL
RQEYRMKLPDAIILATAQIYGFELVTRNTRDGVITPYQP

Nucleotide

• Length: 360 bp

>T193663 NZ_CP093068:3741937-3742155 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9256.53 Da
• Isoelectric Point: 4.3774

>AT193663 WP_001275524.1 NZ_CP070284:c4563796-4563554 [Escherichia albertii]
MSAMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVDQYLENQSKTTISSALGIWQGCEEDGVEYQRKLREEW

Nucleotide

• Length: 243 bp

>AT193663 NZ_CP093068:3741537-3741911 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: B1EQU6