Detailed information of TA system TA193645

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 1612232..1612836
• Replicon: chromosome
• Accession: NZ_CP070284
• Organism: Escherichia albertii strain Sample 169

Toxin (Protein)

• Gene name: doc
• Uniprot ID: B1EM00
• Locus tag: JRC46_RS07750
• Protein ID: WP_000638401.1
• Coordinates: 1612450..1612836 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: JRC46_RS07745
• Protein ID: WP_204620194.1
• Coordinates: 1612232..1612453 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JRC46_RS07725 (1608379)	1608379..1609293	+	915	WP_024164792.1	bestrophin family protein	-
JRC46_RS07730 (1609297)	1609297..1610055	-	759	WP_054410601.1	trans-aconitate 2-methyltransferase	-
JRC46_RS25060 (1610376)	1610376..1610870	-	495	WP_244259603.1	hypothetical protein	-
JRC46_RS07740 (1611085)	1611085..1611892	-	808	Protein_1533	helix-turn-helix domain-containing protein	-
JRC46_RS07745 (1612232)	1612232..1612453	+	222	WP_204620194.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JRC46_RS07750 (1612450)	1612450..1612836	+	387	WP_000638401.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
JRC46_RS25065 (1612916)	1612916..1613983	-	1068	WP_279632101.1	autotransporter outer membrane beta-barrel domain-containing protein	-
JRC46_RS25485 (1613980)	1613980..1614465	-	486	WP_279632102.1	autotransporter outer membrane beta-barrel domain-containing protein	-
JRC46_RS07755 (1614462)	1614462..1617686	-	3225	WP_244259604.1	ESPR-type extended signal peptide-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1578304..1621275	42971
	-	flank	IS/Tn	-	-	1611085..1611471	386

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (3-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-55)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14505.49 Da
• Isoelectric Point: 8.0833

>T193645 WP_000638401.1 NZ_CP070284:1612450-1612836 [Escherichia albertii]
MIWVSAQEVIAFHDRILQRFPGVAGLTDPGRAQALIYRVQNRVHYEGVTDLFELAATYWVAIARGHIFHDGNKRTAFFVT
MTFLWRNGIRIRDVDNSLENLTVEAATGEKTVGQLARCLRERVDSSGN

Nucleotide

• Length: 387 bp

>T193645 NZ_CP093067:67239-67589 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8066.97 Da
• Isoelectric Point: 4.9731

>AT193645 WP_204620194.1 NZ_CP070284:1612232-1612453 [Escherichia albertii]
MQTYTSTQARANIATVLDTALNGEPVEITRRDGSSAVLISKAAFEAYQNAKLDAEFNAIMQRHEHTIEALTDR

Nucleotide

• Length: 222 bp

>AT193645 NZ_CP093067:66953-67234 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: A0A3T5VDW2