Detailed information of TA system TA193632

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 42372..42919
• Replicon: plasmid pESA135_2
• Accession: NZ_CP070283
• Organism: Escherichia albertii strain Sample 170

Toxin (Protein)

• Gene name: parE
• Uniprot ID: G7QEJ9
• Locus tag: JRC42_RS24235
• Protein ID: WP_001384452.1
• Coordinates: 42641..42919 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: JRC42_RS24230
• Protein ID: WP_204620402.1
• Coordinates: 42372..42641 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JRC42_RS24200 (38131)	38131..38730	+	600	WP_204620398.1	PIN domain-containing protein	-
- (39335)	39335..39396	-	62	NuclAT_1	-	-
- (39335)	39335..39396	-	62	NuclAT_1	-	-
- (39335)	39335..39396	-	62	NuclAT_1	-	-
- (39335)	39335..39396	-	62	NuclAT_1	-	-
JRC42_RS24205 (39440)	39440..39589	+	150	WP_244259636.1	Hok/Gef family protein	-
JRC42_RS24210 (39873)	39873..40130	+	258	WP_000083834.1	replication regulatory protein RepA	-
JRC42_RS25565 (40365)	40365..40439	+	75	WP_064763977.1	RepA leader peptide Tap	-
JRC42_RS24220 (40432)	40432..41454	+	1023	WP_204620400.1	plasmid replication initiator RepA	-
JRC42_RS24225 (42159)	42159..42317	+	159	WP_204620401.1	hypothetical protein	-
JRC42_RS24230 (42372)	42372..42641	+	270	WP_204620402.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
JRC42_RS24235 (42641)	42641..42919	+	279	WP_001384452.1	type II toxin-antitoxin system toxin YacB	Toxin
JRC42_RS24240 (42947)	42947..43009	+	63	Protein_43	3'-5' exonuclease	-
JRC42_RS24245 (43098)	43098..43382	+	285	WP_204620403.1	ribbon-helix-helix domain-containing protein	-
JRC42_RS24250 (43382)	43382..43642	+	261	Protein_45	type II toxin-antitoxin system RelE/ParE family toxin	-
JRC42_RS24255 (43738)	43738..43995	+	258	WP_204620446.1	hypothetical protein	-
JRC42_RS24260 (43997)	43997..44692	+	696	WP_204678096.1	hypothetical protein	-
JRC42_RS24265 (44696)	44696..45157	+	462	WP_204678098.1	hypothetical protein	-
JRC42_RS24270 (45422)	45422..45960	-	539	Protein_49	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	espC / espC	1..176494	176494

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10762.38 Da
• Isoelectric Point: 8.4615

>T193632 WP_001384452.1 NZ_CP070283:42641-42919 [Escherichia albertii]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T193632 NZ_CP093043:c1022406-1022074 [Bartonella krasnovii]
ATGAAGCGGGGAGAGATTTGGCTTGTATCTCTTGACCCATCTTCGGGTTATGAACAAAAAGGAACGCGTCCTGTACTGAT
TGTATCACCAGAAGCGTTTAATCGTGTAACGAAAACACCTATTGTCTTGCCTATTACAAGCGGGGGGAATTTTGCTAGAA
CAGCAGGTTTTGCTGTCTCATTAATGGGAGTAGGATTGCATACAACAGGCGTGATACGTTGTGACCAGCCACGTGCTCTT
GATATTGGAGCGCGTCAGGGTAAAAAAATGGAAACAGTTCCCCCTATGATAATGAACGAAGTTCTTGCTAAGCTAGCAAC
ATTTCTCACGTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10265.63 Da
• Isoelectric Point: 8.8736

>AT193632 WP_204620402.1 NZ_CP070283:42372-42641 [Escherichia albertii]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHNMVESSAAAWRD
EMSRKIAGK

Nucleotide

• Length: 270 bp

>AT193632 NZ_CP093043:c1022663-1022406 [Bartonella krasnovii]
ATGTATACAACAAAATTACGTAAAGTTGGAGGGTCAGTGATGCTTTCTATACCACCTGTCTTGCTTGATGTTCTCCATCT
TACTGAAAACACACAAGTGGGCTTGGCTGTTGATAATGGACAATTGATTGTGAAGCCACAAACATTTCCAAGTTACACTC
TTGATGAATTATTAGCCCAATGTAATCCTTCAGTTGATTTTGCGGATGAGGATATAGAGTGGTTTGATGCTCAACCTGTT
GGTAGCGAGCTTTTGTAA

Structures

Toxin

• AlphaFold DB: A0A829CL36