Detailed information of TA system TA193627

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4563196..4563797
• Replicon: chromosome
• Accession: NZ_CP070281
• Organism: Escherichia albertii strain Sample 170

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JRC42_RS22410
• Protein ID: WP_204619762.1
• Coordinates: 4563196..4563555 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B1EQU6
• Locus tag: JRC42_RS22415
• Protein ID: WP_001275524.1
• Coordinates: 4563555..4563797 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JRC42_RS22395 (4560699)	4560699..4561601	+	903	WP_204619761.1	formate dehydrogenase subunit beta	-
JRC42_RS22400 (4561598)	4561598..4562233	+	636	WP_000829019.1	formate dehydrogenase cytochrome b556 subunit	-
JRC42_RS22405 (4562230)	4562230..4563159	+	930	WP_000027704.1	formate dehydrogenase accessory protein FdhE	-
JRC42_RS22410 (4563196)	4563196..4563555	-	360	WP_204619762.1	type II toxin-antitoxin system VapC family toxin	Toxin
JRC42_RS22415 (4563555)	4563555..4563797	-	243	WP_001275524.1	CopG family transcriptional regulator	Antitoxin
JRC42_RS22420 (4564015)	4564015..4564233	-	219	WP_001251292.1	CopG family transcriptional regulator	-
JRC42_RS22425 (4564648)	4564648..4564926	-	279	WP_001296612.1	hypothetical protein	-
JRC42_RS22430 (4564988)	4564988..4565200	-	213	WP_000197774.1	hypothetical protein	-
JRC42_RS22435 (4565285)	4565285..4565575	-	291	WP_000356397.1	NadS family protein	-
JRC42_RS22440 (4565576)	4565576..4565887	-	312	WP_000897302.1	hypothetical protein	-
JRC42_RS22445 (4566116)	4566116..4567024	+	909	WP_204619763.1	alpha/beta hydrolase	-
JRC42_RS22450 (4567088)	4567088..4568029	-	942	WP_204620272.1	bifunctional GNAT family N-acetyltransferase/hotdog fold thioesterase	-
JRC42_RS22455 (4568074)	4568074..4568511	-	438	WP_000560977.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4541012..4565887	24875

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-110)

Antitoxin

• Ribbon-helix-helix protein, CopG (15-48)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13496.66 Da
• Isoelectric Point: 9.4803

>T193627 WP_204619762.1 NZ_CP070281:c4563555-4563196 [Escherichia albertii]
MAKRTALFDTNILIDLFSGRIEAKQALEAWPLQNAISLVTWMEVLVGAKKYNQEHRTRIAMSAFNVIGISQDIAERSVAL
RQEYRMKLPDAIILATAQIYGFELVTRNTRDGVITPYQP

Nucleotide

• Length: 360 bp

>T193627 NZ_CP093042:c854071-853787 [Bartonella krasnovii]
ATGAAAATAACCCCATTTAAACCAGAAGAATATCTTAAAGACATTGAGTCACAGAAAATTTTTCTCAATGAAGCCCTTAA
AACCGGTGATGCCGCCCACATTGCCGATGCTCTTGGTATCGTTGCTCGTGCCCAAAATATGAGTGCCTTAGCAAAAGAAA
CCAATCGCGAACGCAGTGGATTATACCGTTCCTTAAGTAAAACGGGTGACCCAAAGCTTTCTACCTTAGTCGCTGTATTA
TCTGCCCTTAATTTACAACTCTCTGTACAATCTTCTACTTCATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9256.53 Da
• Isoelectric Point: 4.3774

>AT193627 WP_001275524.1 NZ_CP070281:c4563797-4563555 [Escherichia albertii]
MSAMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVDQYLENQSKTTISSALGIWQGCEEDGVEYQRKLREEW

Nucleotide

• Length: 243 bp

>AT193627 NZ_CP093042:c854360-854052 [Bartonella krasnovii]
ATGATACTCATTCATAAAACAATAGAATTTGATACTTGGCTTAAGAAACTTAAAGATAAAAGCGTTAAGGCTATTATTCT
TCAACGTGTTGTACGCTTAAAACAAGGACTTTTCGGTGATGTTAAATTCTTTCATGGCATAGGGGAATTACGTATCCACT
ATGGTGCTGGTTACAGAATTTATTTTACCCAAAAAGGCTCTGATTTTATCCTTTTGTTATGCGGTGGAGATAAATCAACG
CAGCAAAGAGATATTGAACAAGCGTTAAAATTAAAAGAGGAGTACAGTGATGAAAATAACCCCATTTAA

Structures

Antitoxin

• AlphaFold DB: B1EQU6