Detailed information of TA system TA193596

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 3964783..3965359
• Replicon: chromosome
• Accession: NZ_CP070232
• Organism: Escherichia coli strain 10R

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: D3GXZ2
• Locus tag: JTF63_RS18955
• Protein ID: WP_001297643.1
• Coordinates: 3965072..3965359 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A829G8Z0
• Locus tag: JTF63_RS18950
• Protein ID: WP_000063156.1
• Coordinates: 3964783..3965085 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JTF63_RS18935	3961379..3963529	+	2151	WP_001295524.1	pyruvate/proton symporter BtsT	-
JTF63_RS18940	3963579..3963782	+	204	WP_000467859.1	YbdD/YjiX family protein	-
JTF63_RS18945	3963793..3964749	+	957	WP_001297640.1	GTPase	-
JTF63_RS18950	3964783..3965085	-	303	WP_000063156.1	BrnA antitoxin family protein	Antitoxin
JTF63_RS18955	3965072..3965359	-	288	WP_001297643.1	BrnT family toxin	Toxin
JTF63_RS18960	3965558..3966255	-	698	Protein_3703	IS1 family transposase	-
JTF63_RS18965	3966312..3966407	+	96	Protein_3704	dienelactone hydrolase family protein	-
JTF63_RS18970	3966553..3967785	+	1233	WP_001380906.1	multidrug efflux MFS transporter MdtM	-
JTF63_RS18975	3967826..3969106	+	1281	WP_001037380.1	DUF445 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3965558..3965767	209

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11295.77 Da
• Isoelectric Point: 6.8821

>T193596 WP_001297643.1 NZ_CP070232:c3965359-3965072 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T193596 NZ_CP093035:c893544-893260 [Bartonella krasnovii]
ATGAAAATAACCCCATTTAAACCAGAAGAATATCTTAAAGACATTGAGTCACAGAAAATTTTTCTCAATGAAGCCCTTAA
AACCGGTGATGCCGCCCACATTGCCGATGCTCTTGGTATCGTTGCTCGTGCCCAAAATATGAGTGCCTTAGCAAAAGAAA
CCAATCGCGAACGCAGTGGATTATACCGTTCCTTAAGTAAAACGGGTGACCCAAAGCTTTCTACCTTAGTCGCTGTATTA
TCTGCCCTTAATTTACAACTCTCTGTACAATCTTCTACTTCATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11521.99 Da
• Isoelectric Point: 9.4646

>AT193596 WP_000063156.1 NZ_CP070232:c3965085-3964783 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT193596 NZ_CP093035:c893833-893525 [Bartonella krasnovii]
ATGATACTCATTCATAAAACAATAGAATTTGATACTTGGCTTAAGAAACTTAAAGATAAAAGCGTTAAGGCTATTATTCT
TCAACGTGTTGTACGCTTAAAACAAGGACTTTTCGGTGATGTTAAATTCTTTCATGGCATAGGGGAATTACGTATCCACT
ATGGTGCTGGTTACAGAATTTATTTTACCCAAAAAGGCTCTGATTTTATCCTTTTGTTATGCGGTGGAGATAAATCAACG
CAGCAAAGAGATATTGAACAAGCGTTAAAATTAAAAGAGGAGTACAGTGATGAAAATAACCCCATTTAA

Structures

Toxin

• AlphaFold DB: A0A829GEV9

Antitoxin

• AlphaFold DB: A0A829G8Z0