Detailed information of TA system TA193589

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2259126..2259652
• Replicon: chromosome
• Accession: NZ_CP070232
• Organism: Escherichia coli strain 10R

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: JTF63_RS10915
• Protein ID: WP_000323025.1
• Coordinates: 2259365..2259652 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: JTF63_RS10910
• Protein ID: WP_000534858.1
• Coordinates: 2259126..2259365 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JTF63_RS10880	2254485..2254832	-	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
JTF63_RS10885	2254829..2255233	-	405	WP_000839179.1	IS66 family insertion sequence hypothetical protein	-
JTF63_RS10890	2255455..2255742	+	288	Protein_2126	hypothetical protein	-
JTF63_RS10895	2256511..2257824	+	1314	WP_000589005.1	ISNCY family transposase	-
JTF63_RS10900	2258261..2258593	-	333	WP_001301033.1	protein FlxA	-
JTF63_RS10905	2258796..2259101	-	306	WP_001326990.1	hypothetical protein	-
JTF63_RS10910	2259126..2259365	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
JTF63_RS10915	2259365..2259652	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
JTF63_RS10920	2259724..2259879	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
JTF63_RS10925	2260096..2260347	+	252	WP_000980994.1	hypothetical protein	-
JTF63_RS10930	2260414..2260692	+	279	WP_012304870.1	hypothetical protein	-
JTF63_RS10935	2260694..2261743	+	1050	WP_001265199.1	DUF968 domain-containing protein	-
JTF63_RS10940	2261757..2262248	+	492	WP_052424683.1	antitermination protein	-
JTF63_RS10945	2262282..2262979	+	698	WP_106423973.1	IS1 family transposase	-
JTF63_RS10950	2263317..2263532	+	216	WP_000839590.1	phage lysis protein EssD	-
JTF63_RS10955	2263537..2263848	+	312	WP_000189916.1	YdfR family protein	-
JTF63_RS10960	2263845..2264378	+	534	WP_001092971.1	lysozyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2231811..2295301	63490
	-	flank	IS/Tn	-	-	2262686..2262979	293

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T193589 WP_000323025.1 NZ_CP070232:2259365-2259652 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T193589 NZ_CP093033:c1671917-1671630 [Bartonella krasnovii]
ATGCTTCCGGTTGTTTGGTTATCTTCGGCTCGCGATGATTTGCGTCAGATTTTAACTTACATTGCTCGTGAAAATCCATC
TGCTGCACGCCGAATGAAAAGGTTTTTGGAAACATCTGTACTGCCATTGGCTGAACATCCATATCTGTACAGACAGAGTG
AGAGGATATTAGGGTTACGGGAAATTATAGCACATCCAAATTACCTTATATTGTATCGAGTAGCTGCAAATCAGATCGAA
ATTGTAGCTGTCGTACATGCCCGACGTGAATTCCCTACCTCTCTTTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT193589 WP_000534858.1 NZ_CP070232:2259126-2259365 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT193589 NZ_CP093033:c1672105-1671917 [Bartonella krasnovii]
ATGAAAAACACCCTTTCTCCGTTCATTTCAGAGTTCGAAACCGTTGAACAGGAAAGCAGCCACACGGCGTGGTTGCGCGA
TAAGGTCGCGATAAGTCTTGCAAACCTATCTCCTCCTATTCCGCATGATGAGGTCATGGCTGAAATGGACGTTATAATCA
GTCAGCTTGAAAATGAACAGAAAAAGTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8